The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice
The OsMPK15 Negatively Regulates Magnaporthe oryza and Xoo Disease Resistance via SA and JA Signaling Pathway in Rice
复制标题
OsMPK15 通过 SA 和 JA 信号通路负调控水稻稻瘟病和 Xoo 病害抗性
DOI：
10.3389/fpls.2019.00752
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发表时间：
2019-06-21
影响因子：
5.6
通讯作者：
Cao, Liyong
中科院分区：
文献类型：
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作者：
Hong, Yongbo;Liu, Qunen;Cao, Liyong
丝裂原活化蛋白激酶（MAPK）级联反应在生物和非生物胁迫的响应中起着重要作用。然而，各种MAPK成员调节水稻植物免疫应答的机制仍然是难以捉摸的。本文通过构建OsMPK 15基因敲除和过表达突变体，研究OsMPK 15基因在不同真菌和细菌接种条件下的抗病性。OsMPK 15基因敲除后，致病相关（PR）基因组成型表达，病原相关分子模式（PAMP）激发子几丁质（chitin）诱导活性氧（ROS）积累增加，显著提高了水稻对稻瘟病菌和黄单胞菌的抗病性。白叶枯病菌（Xoo），分别引起稻瘟病和白叶枯病。相反，在OsMPK 15-OE中，PR基因和ROS的表达量均下降。同时，植物激素如水杨酸（SA）和茉莉酸（JA）在mpk 15突变体系中积累，而在OsMPK 15-OE系中减少。SA和JA途径相关基因的表达在mpk 15突变体中显著上调，而在OsMPK 15-OE系中下调。OsMPK 15可能通过调节SA和JA介导的信号通路负调控抗病性。
Mitogen-activated protein kinase (MAPK) cascades play central roles in response to biotic and abiotic stresses. However, the mechanisms by which various MAPK members regulate the plant immune response in rice remain elusive. In this article, to characterize the mechanisms, the knock-out and overexpression mutants of OsMPK15 were constructed and the disease resistance was investigated under the various fungal and bacterial inoculations. The knock-out mutant of OsMPK15 resulted in the constitutive expression of pathogenesis-related (PR) genes, increased accumulation of reactive oxygen species (ROS) triggered by the pathogen-associated molecular pattern (PAMP) elicitor chitin, and significantly enhanced the disease resistance to different races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo), which cause the rice blast and bacterial blight diseases, respectively. On contrary, the expression of PR genes and ROS were down-regulated in the OsMPK15-overexpressing (OsMPK15-OE) lines. Meanwhile, phytohormones such as salicylic acid (SA) and jasmonic acid (JA) were accumulated in the mpk15 mutant lines but decreased in the OsMPK15-OE lines. The expression of SA- and JA-pathway associated genes were significantly upregulated in the mpk15 mutant, whereas it was down regulated in the OsMPK15-OE lines. We conclude that OsMPK15 may negatively regulate the disease resistance through modulating SA- and JA-mediated signaling pathway.