Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth
Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth
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DOI：
10.1016/s0014-5793(02)03398-7
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发表时间：
2002-10-23
期刊：
影响因子：
3.5
通讯作者：
Kinoshita, Y
中科院分区：
文献类型：
--
作者：
Kadowaki, Y;Ishihara, S;Kinoshita, Y
Reg 是一种对胰腺β细胞和胃干细胞具有促有丝分裂作用的生长因子。迄今为止，还没有关于Reg介导的细胞内信号转导途径的信息。 Reg 在胃癌发生中的作用尚不清楚。在当前的研究中，检查了胃癌细胞中的Reg信号通路。 Reg 处理 MKN45 胃癌细胞导致多种细胞蛋白酪氨酰磷酸化，并随后激活经典 MAPK、ERK1/2。 Reg 还以剂量依赖性方式刺激 MKN45 和 AGS 胃癌细胞中的胸苷掺入。最后，Reg被证明在体内大量胃癌中高表达。综上所述，这些数据表明胃癌细胞已经获得了过度表达Reg蛋白的能力，这赋予了它们自身额外的增殖能力，从而产生了相当大的生长优势。 (C) 2002 年欧洲生化学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Reg is a growth factor with mitogenic effects on pancreatic beta cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.