Epithelial Cells Lining Salivary Gland Ducts Are Early Target Cells of Severe Acute Respiratory Syndrome Coronavirus Infection in the Upper Respiratory Tracts of Rhesus Macaques
Epithelial Cells Lining Salivary Gland Ducts Are Early Target Cells of Severe Acute Respiratory Syndrome Coronavirus Infection in the Upper Respiratory Tracts of Rhesus Macaques
复制标题
DOI：
10.1128/jvi.02292-10
复制
发表时间：
2011-04-01
影响因子：
5.4
通讯作者：
Chen, Zhiwei
中科院分区：
文献类型：
--
作者：
Liu, Li;Wei, Qiang;Chen, Zhiwei
严重急性呼吸综合征冠状病毒(SARS-CoV)在病毒传播中起着至关重要的作用。然而，唾液中高病毒载量的来源仍然难以捉摸。在这里，我们研究了中国猕猴鼻腔接种单循环伪型病毒和致病性SARS冠状病毒后，整个呼吸道组织中的早期感染靶细胞。我们发现血管紧张素转换酶2阳性(ACE2(+))细胞广泛分布于上呼吸道，唾液腺导管内的血管紧张素转换酶2(+)上皮细胞是感染的早期靶细胞。我们的发现对SARS-CoV的早期诊断和预防也有一定的意义。
The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2(+)) cells were widely distributed in the upper respiratory tract, and ACE2(+) epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.