METABOLISM OF THE LIPID-PEROXIDATION PRODUCT 4-HYDROXYNONENAL BY ISOLATED HEPATOCYTES AND BY LIVER CYTOSOLIC FRACTIONS
METABOLISM OF THE LIPID-PEROXIDATION PRODUCT 4-HYDROXYNONENAL BY ISOLATED HEPATOCYTES AND BY LIVER CYTOSOLIC FRACTIONS
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DOI：
10.1042/bj2280363
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
LANG, J
中科院分区：
文献类型：
--
作者：
ESTERBAUER, H;ZOLLNER, H;LANG, J
研究了脂质过氧化产物4-羟基壬烯醛和其他几种相关醛类在离体肝细胞和大鼠肝亚细胞组分中的代谢。肝细胞在不依赖于O2的过程中快速代谢4-羟基壬烯醛，最大速率（取决于细胞制备）为130-230 nmol/min/106个细胞（平均193 ± 100 nmol/min）。50）。醛也被补充有NADH的整个大鼠肝匀浆和胞质部分（140，000 g上清液）快速利用，而补充有NADH的纯化的细胞核、线粒体和微粒体显示醛没有显著消耗。在胞质溶胶中，NADH介导的醛代谢表现出1：1的化学计量，即，1摩尔氧化的NADH/摩尔消耗的羟基壬烯醛，醛的表观Km值为0.1 mM。添加吡唑（10 mM）或细胞质的热灭活完全废除了醛代谢。各种研究结果强烈表明，肝细胞和大鼠肝细胞液，分别转化4-羟基壬烯醛酶转化为相应的醇，壬-2-烯-1，4-二醇。
The metabolism of the lipid peroxidation product 4-hydroxynonenal and of several other related aldehydes by isolated hepatocytes and rat liver subcellular fractions was investigated. Hepatocytes rapidly metabolize 4-hydroxynonenal in an O2-independent process with a maximum rate (depending on cell preparation) ranging from 130-230 nmol/min per 106 cells (average 193 .+-. 50). The aldehyde is also rapidly utilized by whole rat liver homogenate and the cytosolic fraction (140,000 g supernatant) supplemented with NADH, whereas purified nuclei, mitochondria and microsomes supplemented with NADH show no noteworthy consumption of the aldehyde. In cytosol, the NADH-mediated metabolism of the aldehyde exhibits a 1:1 stoichiometry, i.e., 1 mol of NADH oxidized/mol of hydroxynonenal consumed, and the apparent Km value for the aldehyde is 0.1 mM. Addition of pyrazole (10 mM) or heat inactivation of the cytosol completely abolishes aldehyde metabolism. The various findings strongly suggest that hepatocytes and rat liver cytosol, respectively, convert 4-hydroxynonenal enzymically to the corresponding alcohol, non-2-ene-1,4-diol.