Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
Maternal Cytomegalovirus Antibodies during Early and Late (Persistent) Phases after Primary Cytomegalovirus Infection during Pregnancy: An Observational Study
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DOI：
10.31083/j.ceog4912269
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发表时间：
2022-12-01
影响因子：
0.2
通讯作者：
Ikeda, Tomoaki
中科院分区：
文献类型：
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作者：
Toriyabe, Kuniaki;Kitamura, Asa;Ikeda, Tomoaki
背景：我们使用我们储存的血清样本，分析了妊娠期间原发性 CMV 感染以及随后从妊娠后期到分娩后很长一段时间内先天性 CMV 感染的母亲的每种巨细胞病毒 (CMV) 抗体的早期和晚期（持续）阶段。方法：我们使用了从患有 CMV 免疫球蛋白 (Ig) G 血清转化和随后婴儿先天性 CMV 感染的母亲在怀孕期间到分娩后获得的储存血清样本。分别使用 Denka IgG 测定、Denka IgM 测定 Ver.1 和 Ver.2、Enzygnost IgG 测定和 Denka IgG 亲和力测定来评估 CMV 抗体，包括 CMV IgG 滴度、IgM 滴度和 IgG 亲和力测定。我们分析了从怀孕后期到产后的长时间内每种CMV抗体的动态以及每种抗体的相关性，计算了皮尔逊相关系数（R2）。结果：我们使用了 2013 年至 2018 年间从 12 名参与者获得的 67 份血清样本。CMV IgG 持续增加直至 61 周，此后没有显着变化。 CMV IgM 一直下降到 52 周，之后在两次检测中都没有显着变化。 CMV IgG 亲和力直到 64 周才增加，之后在两次检测中都没有显着变化。在 CMV IgM 中，两种不同的 IgM 检测之间存在很强的正相关性 (R2 = 0.9326)。后期（60周后）的血清结果被归入高CMV IgG亲合力和低CMV IgM滴度的区域，这可能相当于持续性IgM。结论：原发感染后期母亲CMV抗体呈高IgG亲合力和低IgM滴度，可能相当于持续性IgM。
Background: We analyzed both early and late (persistent) phases of each cytomegalovirus (CMV) antibody in mothers with primary CMV infection during pregnancy and subsequent congenital CMV infection for a long period from late pregnancy to after delivery using our stored serum samples. Methods: We used stored serum samples obtained during pregnancy to after delivery from mothers with CMV immunoglobulin (Ig) G seroconversion and subsequent infant congenital CMV infection. CMV antibodies, including CMV IgG titer, IgM titer, and IgG avidity, were assessed using the Denka IgG assay, Denka IgM assay Ver.1 and Ver.2, and Enzygnost IgG assay and Denka IgG avidity assay, respectively. We analyzed the dynamics of each CMV antibody for a long period from late pregnancy to after delivery and correlations of each antibody, calculating Pearson's correlation coefficients (R2). Results: We used 67 serum samples obtained from 12 included participants between 2013 and 2018. CMV IgG increased until 61 weeks and did not change significantly after. CMV IgM decreased until 52 weeks and did not change significantly after that in both assays. CMV IgG avidity increased until 64 weeks and did not change significantly after that in both assays. In CMV IgM, a strong positive correlation was found (R2 = 0.9326) between the two different IgM assays. Serum results of the late phase (after 60 weeks) were subsumed into the area of high CMV IgG avidity and low CMV IgM titer, which probably was equivalent to the persistent IgM. Conclusions: CMV antibodies in mothers during the late phase of primary infection were in high IgG avidity and low IgM titer, which probably was equivalent to the persistent IgM.