Pulmonary Delivery of Proteins Using Nanocomposite Microcarriers
Pulmonary Delivery of Proteins Using Nanocomposite Microcarriers
复制标题
DOI：
10.1002/jps.24681
复制
发表时间：
2015-12-01
影响因子：
3.8
通讯作者：
Saleem, Imran Y.
中科院分区：
文献类型：
--
作者：
Alfagih, Iman;Kunda, Nitesh;Saleem, Imran Y.
在本研究中，采用Taguchi设计确定了以可生物降解聚合物聚己二酸甘油-共omega-pentadecalactone （PGA-co-PDL）为原料制备牛血清白蛋白（BSA）负载纳米颗粒（NPs）的最佳工艺参数。以牛血清白蛋白（BSA）为模型蛋白，采用双乳液蒸发工艺，将亮氨酸喷雾干燥，制备纳米复合微粒（NCMPs）。研究了不同参数对树突状细胞（DC）摄取和毒性的影响。研究了NCMPs的形态、产率、雾化、体外释放行为和BSA结构。NP的大小主要受聚合物质量和5 mg/mL浓度下细胞存活率为85%的小粒径4 h的影响。这些结果表明，制备适合吸入的载蛋白PGA-co-PDL NCMPs的最佳工艺参数。(C) 2015 Wiley期刊公司和美国药剂师协会
In this study, Taguchi design was used to determine optimal parameters for the preparation of bovine serum albumin (BSA)-loaded nanoparticles (NPs) using a biodegradable polymer poly(glycerol adipate-co-omega-pentadecalactone) (PGA-co-PDL). NPs were prepared, using BSA as a model protein, by the double emulsion evaporation process followed by spray-drying from leucine to form nanocomposite microparticles (NCMPs). The effect of various parameters on NP size and BSA loading were investigated and dendritic cell (DC) uptake and toxicity. NCMPs were examined for their morphology, yield, aerosolisation, in vitro release behaviour and BSA structure. NP size was mainly affected by the polymer mass used and a small particle size 85% cell viability at 5 mg/mL concentration after 4 h. These results indicate the optimal process parameters for the preparation of protein-loaded PGA-co-PDL NCMPs suitable for inhalation. (C) 2015 Wiley Periodicals, Inc. and the American Pharmacists Association