Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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DOI：
10.3791/50466
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发表时间：
2013-03-01
影响因子：
1.2
通讯作者：
Berggren, Per-Olof
中科院分区：
文献类型：
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作者：
Abdulreda, Midhat H.;Caicedo, Alejandro;Berggren, Per-Olof
活体成像已成为生物学研究中不可或缺的工具。在这个过程中，许多成像技术已经被开发出来，以非侵入性地研究动物的不同生物过程。然而，现有活体成像模式的主要技术限制是不能将联合收割机非侵入性纵向成像与单细胞分辨率能力相结合。我们在这里展示了移植到眼前房中如何规避这种显著的限制，提供了一个多功能的实验平台，可以在体内实现具有细胞分辨率的非侵入性纵向成像。我们证明了移植过程中的小鼠，并提供了具有代表性的结果，使用具有临床意义的模型，即胰岛移植。除了能够直接可视化移植到眼前房的各种组织外，这种方法还提供了一个平台，通过在靶组织中进行长期随访和监测来筛选药物。由于其多功能性，将组织/细胞移植到眼前房中不仅有利于移植治疗，而且还扩展到其他体内应用，以研究生理和病理生理过程，如信号转导和癌症或自身免疫性疾病的发展。
Intravital imaging has emerged as an indispensable tool in biological research. In the process, many imaging techniques have been developed to study different biological processes in animals non-invasively. However, a major technical limitation in existing intravital imaging modalities is the inability to combine non-invasive, longitudinal imaging with single-cell resolution capabilities. We show here how transplantation into the anterior chamber of the eye circumvents such significant limitation offering a versatile experimental platform that enables non-invasive, longitudinal imaging with cellular resolution in vivo. We demonstrate the transplantation procedure in the mouse and provide representative results using a model with clinical relevance, namely pancreatic islet transplantation. In addition to enabling direct visualization in a variety of tissues transplanted into the anterior chamber of the eye, this approach provides a platform to screen drugs by performing long-term follow up and monitoring in target tissues. Because of its versatility, tissue/cell transplantation into the anterior chamber of the eye not only benefits transplantation therapies, it extends to other in vivo applications to study physiological and pathophysiological processes such as signal transduction and cancer or autoimmune disease development.