Time-resolved fluorescence polarization from ordered biological assemblies.
Time-resolved fluorescence polarization from ordered biological assemblies.
复制标题
来自有序生物组件的时间分辨荧光偏振。
DOI：
10.1016/s0006-3495(85)83818-2
复制
发表时间：
1985
影响因子：
3.4
通讯作者：
Burghardt,TP
中科院分区：
文献类型：
--
作者：
Burghardt,TP
我们计算的时间依赖性的偏振荧光信号从荧光标记的生物组件的旋转扩散在一个任意的三维角电位的元素。我们已经使用角势的模型无关描述来公式化该计算，其中角势通过在具有由时间无关方法确定的展开系数（或阶参数）的正交函数的完整集合中的展开来描述（Burghardt，T. P.，1984，Biopolymers，23：2382-2406）。我们已经将计算应用于放松的肌纤维中的荧光标记肌球蛋白跨桥。在一篇相关的论文中，我们描述了肌球蛋白交叉桥在角势中旋转扩散的实验观察（Burghardt，T。P.，和N. L. Thompson，1985，Biochemistry，24：3731-3735）。
We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential wherein the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T. P., 1984, Biopolymers, 23:2382–2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridges rotationally diffusing in an angular potential (Burghardt, T. P., and N. L. Thompson, 1985, Biochemistry, 24:3731–3735).