Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia
Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia
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DOI：
10.3354/dao02612
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发表时间：
2013-07-09
影响因子：
1.4
通讯作者：
De Vico, Gionata
中科院分区：
文献类型：
--
作者：
Carella, Francesca;Figueras, Antonio;De Vico, Gionata
贫血性肿瘤（HN）是一种病理条件报告的几个双壳类在不同的地理区域。在这项研究中，我们描述的细胞形态学特征和增殖行为，增殖细胞核抗原（PCNA），HN在常见的皱皮角皮病和地中海贻贝贻贝评估。在贻贝中检测到至少5种类型的非典型血细胞，包括A型和B型细胞，先前在M. edulis和Mytilus sp.，在疾病的早期阶段A型细胞占优势，在更晚期阶段B型细胞占优势。PCNA免疫组化阳性率为97 ~ 100%，既有胞浆型（A细胞），又有核型（B细胞）。相反，在C.然而，没有明显的形态学细胞亚群，用PCNA染色的非典型血细胞（范围93 - 100%）显示在疾病的早期阶段核表达和在更晚期阶段胞质表达。以上结果表明，不同的组织病理学途径的HN在贻贝和共同的蛤蚌。
Haemic neoplasia (HN) is a pathologic condition reported in several bivalve species in different geographic areas. In this study we describe the cytomorphological features and the proliferative behaviour, assessed by the proliferating cell nuclear antigen (PCNA), of HN in common cockle Cerastoderma edule and Mediterranean mussel Mytilus galloprovicialis. In mussels the presence of at least 5 types of atypical haemocytes was detected, including A- and B-type cells, previously described in M. edulis and Mytilus sp., with predominance of A-type cells in early phases of the disease and B-type cells in more advanced stages. PCNA immunostaining was positive for 97 to 100% of the neoplastic cells, with both cytoplasmic (A cells) and nuclear patterns (B cells). Conversely, in C. edule there was no distinctive morphological cell sub-population, and staining atypical haemocytes with PCNA (range 93 to 100%) showed nuclear expression in early phases of disease and cytoplasmic expression in more advanced stages. The above findings suggest distinct histo-pathogenetic pathways for HN in mussels and common cockles.