Sendai virus induces various cytokines in human peripheral blood leukocytes: different susceptibility of cytokine molecules to low pH.
Sendai virus induces various cytokines in human peripheral blood leukocytes: different susceptibility of cytokine molecules to low pH.
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仙台病毒在人外周血白细胞中诱导多种细胞因子：细胞因子分子对低pH值的敏感性不同。
DOI：
10.1006/cyto.1998.0411
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发表时间：
1999
期刊：
影响因子：
3.8
通讯作者：
R. Mažuran
中科院分区：
文献类型：
--
作者：
S. Židovec;R. Mažuran
细胞培养物的病毒感染诱导各种细胞因子的合成，这些细胞因子可以抑制或刺激病毒复制。仙台病毒在体外诱导人外周血白细胞（hPBL）中产生大量具有生物活性的干扰素（IFN-alphan3）以及许多其他细胞因子。仙台病毒感染的 hPBL 上清液含有生物活性 IFN-αn3、大量免疫原性 IFN-γ、单核因子（IL-1α、IL-β、TNF-α）、淋巴因子（IL-6、TNF-β）、生长因子（PDGF-AB）以及小浓度的 IL-2 和 GM-CSF。降低pH 2.0灭活仙台病毒对细胞因子浓度的影响分析表明，IL-1α、TNF-α、TNF-β和IFN-γ对酸性条件敏感，而IFN-αn3、IL-1β、IL-6和IL-2浓度保持不变。
Virus infection of cell cultures induces the synthesis of various cytokines which can either inhibit or stimulate virus replication. The Sendai virus induces large quantities of biologically active interferon (IFN-alphan3) in human peripheral blood leukocytes (hPBL) in vitro, as well as many other cytokines. The supernatants of Sendai virus-infected hPBL contained biologically active IFN-alphan3, significant amounts of immunogenic IFN-gamma, monokines (IL-1alpha, IL-beta, TNF-alpha), lymphokines (IL-6, TNF-beta), growth factor (PDGF-AB) and small concentrations of IL-2 and GM-CSF. The analysis of the influence of the Sendai virus inactivation by lowering pH 2.0 on the cytokine concentrations showed that IL-1alpha, TNF-alpha, TNF-beta and IFN-gamma are susceptible to acid conditions, while IFN-alphan3, IL-1beta, IL-6 and IL-2 concentrations remained unchanged.