Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA and wbpI and their Escherichia coli homologues wecC and wecB are not functionally interchangeable
Pseudomonas aeruginosa B-band lipopolysaccharide genes wbpA and wbpI and their Escherichia coli homologues wecC and wecB are not functionally interchangeable
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DOI：
10.1111/j.1574-6968.2000.tb09219.x
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发表时间：
2000-08-15
影响因子：
2.1
通讯作者：
Lam, JS
中科院分区：
文献类型：
--
作者：
Burrows, LL;Pigeon, KE;Lam, JS
铜绿假单胞菌血清型O 5的O抗原单位是一种复合三糖，含有2-乙酰氨基-3-乙酰亚胺基-2，3-双脱氧-β-D-甘露糖醛酸、2-乙酰亚胺基-3-乙酰亚胺基-2，3-双脱氧-β-D-甘露糖醛酸和2-乙酰亚胺基-2，6-脱氧-β-D-半乳糖胺。推定的UDP-D-N-乙酰葡糖胺（UDP-D-GlcNAc）差向异构酶基因wbpI或推定的UDP-D-N-乙酰甘露糖胺脱氢酶基因wbpA中的特定敲除突变导致菌株不再产生B带脂多糖，证实了这些基因在B带O抗原合成中的重要作用。尽管wbpI和wbpA与参与肠细菌共同抗原合成的大肠杆菌基因wecB（rffE）和wecC（vffD）有大约50%的相似性，但交叉互补实验并不成功。这些结果表明，铜绿假单胞菌UDP-D-GlcNAc前体可能在进一步修饰之前被二-N-乙酰化，从而阻止了E.大肠杆菌的酶将其识别为底物。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The O antigen unit of Pseudomonas aeruginosa serotype O5 is a complex trisaccharide containing 2-acetamido-3-acetiminido-2,3-dideoxy-beta-D-mannuronic acid, 2-acetimido-3-acetimido-2,3-dideoxy-beta-D-mannuronic acid, and 2-acetimido-2,6-deoxy-beta-D-galactosamine. Specific knockout mutations in the putative UDP-D-N-acetylglucosamine (UDP-D-GlcNAc) epimerase gene, wbpI or the putative UDP-D-N-acetylmannosamine dehydrogenase gene, wbpA, resulted in strains that no longer produced B-band lipopolysaccharide, confirming the essential roles of these genes in B-band O antigen synthesis. Despite approximately 50% similarity of wbpI and wbpA to the Escherichia coli genes wecB (rffE) and wecC (vffD) involved in enterobacterial common antigen synthesis, cross-complementation experiments were not successful. These results imply that the P. aeruginosa UDP-D-GlcNAc precursor may be di-N-acetylated prior to further modification, preventing the E. coli enzymes from recognizing it as a substrate. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.