Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance
Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance
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DOI：
10.1016/j.fct.2015.05.009
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发表时间：
2015-09-01
影响因子：
4.3
通讯作者：
Bodin, Johanna
中科院分区：
文献类型：
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作者：
Nygaard, Unni Cecilie;Vinje, Nina Eriksen;Bodin, Johanna
在呼吸道过敏、食物过敏和口服耐受的小鼠模型中研究了生命早期通过饮用水暴露于双酚A（BPA）的影响。将Balb/c小鼠暴露于BPA（0、10或100 μ g/ml），并将后代鼻内暴露于过敏原卵清蛋白（OVA）。在暴露于BPA（0、1、10或100 μ g/ml）后，通过灌胃用食物过敏原羽扇豆致敏C3 H/HeJ后代。在单独的后代中，在进入食物过敏诱导方案前一周通过管饲5 mg羽扇豆诱导口服耐受。在气道过敏模型中，BPA（100 μ g/ml）引起支气管肺泡灌洗液（BALF）中嗜酸性粒细胞数量增加，并有增加OVA特异性IgE水平的趋势。在食物过敏和耐受模型中，BPA没有改变临床过敏反应或抗体反应，但诱导脾细胞细胞因子的改变和小鼠肥大细胞蛋白酶（MMCP）-1血清水平降低。总之，生命早期通过饮用水暴露于BPA仅在高剂量下适度增加气道过敏小鼠模型的过敏反应，而不是食物过敏和耐受性小鼠模型。因此，我们的数据不支持BPA在与人类相关的暴露水平下促进过敏发展。(C)2015爱思唯尔有限公司版权所有。
The impact of early life exposure to bisphenol A (BPA) through drinking water was investigated in mouse models of respiratory allergy, food allergy and oral tolerance. Balb/c mice were exposed to BPA (0, 10 or 100 mu g/ml), and the offspring were intranasally exposed to the allergen ovalbumin (OVA). C3H/HeJ offspring were sensitized with the food allergen lupin by intragastric gavage, after exposure to BPA (0, 1, 10 or 100 mu g/ml). In separate offspring, oral tolerance was induced by gavage of 5 mg lupin one week before entering the protocol for the food allergy induction. In the airway allergy model, BPA (100 mu g/ml) caused increased eosinophil numbers in bronchoalveolar lavage fluid (BALF) and a trend of increased OVA-specific IgE levels. In the food allergy and tolerance models, BPA did not alter the clinical anaphylaxis or antibody responses, but induced alterations in splenocyte cytokines and decreased mouse mast cell protease (MMCP)-1 serum levels. In conclusion, early life exposure to BPA through drinking water modestly augmented allergic responses in a mouse model of airway allergy only at high doses, and not in mouse models for food allergy and tolerance. Thus, our data do not support that BPA promotes allergy development at exposure levels relevant for humans. (C) 2015 Elsevier Ltd. All rights reserved.