Therapeutic effect of a traditional Chinese medicine, ren-shen-yang-rong-tang (Japanese name: Ninjin'yoeito) on nitric oxide-mediated lung injury in a mouse infected with murine cytomegalovirus
Therapeutic effect of a traditional Chinese medicine, ren-shen-yang-rong-tang (Japanese name: Ninjin'yoeito) on nitric oxide-mediated lung injury in a mouse infected with murine cytomegalovirus
复制标题
DOI：
10.1016/j.intimp.2005.10.011
复制
发表时间：
2006-04-01
影响因子：
5.6
通讯作者：
Sawamura, S
中科院分区：
文献类型：
--
作者：
Tanaka, K;Sawamura, S
观察中药人肾养荣汤对小鼠巨细胞病毒相关性肺炎的治疗作用。在巨细胞病毒肺炎中，干扰素-γ诱导的一氧化氮(NO)参与了其发病机制。给28天前感染MCMV 0.2LD(50)的小鼠腹腔注射抗CD3单抗，可诱发小鼠肺内MCMV肺炎，并在肺内积聚大量产生干扰素-γ的细胞，同时伴有血清NO水平升高和诱导型一氧化氮合酶(INOS)mRNA的大量表达，最终导致小鼠死亡。单抗注射当天和次日分别灌胃给予NYT(1000 mg/kg/d)，可抑制iNOS-mRNA的表达，降低血清NO水平。存活率从0%提高到57.1%。接受NYT治疗的小鼠的肺部病理结果与未感染对照的小鼠相似。相比之下，NYT本身既不影响产生干扰素-γ的细胞比率，也不影响MCMV滴度。结果表明，NYT对MCMV肺炎有一定的治疗作用，其机制可能是通过减轻干扰素-γ诱导的NO所致的炎症程度来实现的。同样有趣的是，只有两次口服NYT对病毒性疾病有治疗效果。(C)2005 Elsevier B.V.保留所有权利。
The therapeutic effect of a traditional Chinese medicine ren-shen-yang-rong-tang (Japanese name: Ninjin'yoeito, NYT) on murine cytomegalovirus (MCNW)-associated pneumonitis was examined. In MCMV-pneumonitis, IFN-gamma-induced nitric oxide (NO) mediates its pathogenesis. When mice, which had been infected with 0.2LD(50) of MCMV at 28 days previously, were intraperitoneally injected with anti-CD3 monoclonal antibody (mAb), MCMV-pneumonitis was induced in the lung, where high amounts of IFN-gamma-producing cells thereafter accumulated, accompanied by an elevation in the NO level in the serum and abundant expression of inducible NO synthase (iNOS) mRNA, thus resulting in all mice eventually dying. When the mice were orally treated with NYT (1000 mg/kg/day) once on the day of mAb injection and once the day after, the expression level of iNOS-mRNA was suppressed and NO level in the serum decreased. The survival rate improved from 0% to 57.1%. The pathological findings of the lungs in the NYT-treated mice were comparable to those of the uninfected controls. In contrast, NYT itself did not affect either the ratio of IFN-gamma-producing cells or MCMV titer. As a result, NYT had a therapeutic effect on MCMV-pneumonitis by decreasing the degree of inflammation mediated by the IFN-gamma-induced NO. It is also interesting to note that only two oral administrations of NYT had a therapeutic effect on viral disease. (c) 2005 Elsevier B.V. All rights reserved.