Characterization of proanthocyanidins from grape seeds
Characterization of proanthocyanidins from grape seeds
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DOI：
10.1016/s0367-326x(99)00161-6
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发表时间：
2000-04-01
期刊：
影响因子：
3.4
通讯作者：
Peterlongo, F
中科院分区：
文献类型：
--
作者：
Gabetta, B;Fuzzati, N;Peterlongo, F
对葡萄籽原花青素(Leucoselect(TM))进行了分析。高效液相色谱-热喷雾质谱仪(TSP-MS)可以检测到单体黄烷-3-醇和二聚体原花青素。Sephadex(R)LH-20树脂分级，并用凝胶渗透色谱(GPC)和电喷雾质谱仪(ESI-MS)对分离出的组分进行分析，从而对Leucoselect(TM)的原花青素成分进行了完整的表征。分析表明，大约15%的(+)-儿茶素(1)和(-)-表儿茶素(2)，(-)-表儿茶素3-O-没食子酸酯(3)的80%，二聚体、三聚体、四聚体及其没食子酸酯，以及5%的五聚体、六聚体、七聚体及其没食子酸酯。(C)2000 Elsevier Science B.V.保留所有权利。
Leucoselect(TM) (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex(R) LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect(TM). The analysis revealed the presence of approximately 15% of (+)-catechin (1) and (-)-epicatechin (2), 80% of (-)-epicatechin 3-O-gallate (3), dimers, trimers, tetramers and their gallates and 5% of pentamers, hexamers, heptamers and their gallates. (C) 2000 Elsevier Science B.V. All rights reserved.