Functionalized self-assembling peptide nanofiber hydrogel as a scaffold for rabbit nucleus pulposus cells.
Functionalized self-assembling peptide nanofiber hydrogel as a scaffold for rabbit nucleus pulposus cells.
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DOI：
10.1002/jbm.a.33300
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Baichuan Wang;Yong-chao Wu;Zengwu Shao;Shuhua Yang;B. Che;Caixia Sun;Zhilin Ma;Yannan Zhang
中科院分区：
文献类型：
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作者：
Baichuan Wang;Yong-chao Wu;Zengwu Shao;Shuhua Yang;B. Che;Caixia Sun;Zhilin Ma;Yannan Zhang
本研究设计了一种新的功能化肽RLN，该肽含有link蛋白氨基末端肽link N的生物活性基序。将RLN肽溶液与RADA16混合，自组装成一种链接N纳米纤维支架（LN-NS）。采用原子力显微镜（AFM）对LN-NS进行表征。并对该纳米纤维支架对兔髓核细胞（NPCs）的生物相容性和生物活性进行了评价。设计的功能化纳米纤维支架具有很小的细胞毒性，并能明显促进npc的粘附。在三维细胞培养实验中，共聚焦重建图像证明功能化的ln - ns引导的npc从表面迁移到水凝胶中，而RADA16支架则没有。此外，功能化的LN-NS显著刺激细胞外基质（ECM）的生物合成。我们的研究结果表明，含有链接N的功能化纳米纤维支架与兔NPCs具有良好的生物相容性和生物活性，可用于髓核再生。
In this study, a new functionalized peptide RLN was designed containing the bioactive motif link N, the amino terminal peptide of link protein. A link N nanofiber scaffold (LN-NS) was self-assembled by mixing peptide solution of RLN and RADA16. The characterization of LN-NS was tested using atomic force microscopy (AFM). The biocompatibility and bioactivity of this nanofiber scaffold for rabbit nucleus pulposus cells (NPCs) were also evaluated. This designer functionalized nanofiber scaffold exhibited little cytotoxicity and promoted NPCs adhesion obviously. In three-dimensional cell culture experiments, confocal reconstructed images testified that the functionalized LN-NS-guided NPCs migration from the surface into the hydrogel considerably, in which the RADA16 scaffold did not. Moreover, the functionalized LN-NS significantly stimulated the biosynthesis of extracelluar matrices (ECM) by NPCs. Our findings demonstrate that the functionalized nanofiber scaffold containing link N had excellent biocompatibility and bioactivity with rabbit NPCs and could be useful in the nucleus pulposus regeneration.