β-sitosterol activates the sphingomyelin cycle and induces apoptosis in LNCaP human prostate cancer cells
β-sitosterol activates the sphingomyelin cycle and induces apoptosis in LNCaP human prostate cancer cells
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DOI：
10.1080/01635589809514709
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Awad, AB
中科院分区：
文献类型：
--
作者：
von Holtz, RL;Fink, CS;Awad, AB
流行病学证据表明，食用含有大量植物产品的低脂、高纤维饮食的男性患前列腺癌的风险低于食用西方饮食的男性。这两种饮食之间的主要区别之一是膳食脂肪的类型，包括膳食固醇。本研究旨在比较两种膳食固醇对体外培养的前列腺癌细胞的作用。比较了β-谷甾醇（SIT）（最常见的植物甾醇）和胆固醇（一种动物甾醇）对LNCaP细胞生长、分化、凋亡和鞘磷脂循环中间产物的影响。将细胞用通过环糊精载体递送的甾醇处理长达7天。相比之下，维生素胆固醇，SIT（16 μ M）减少了24%的增长和诱导凋亡的四倍，这是伴随着细胞变圆和神经酰胺生产增加50%。通过前列腺特异性抗原和前列腺酸性磷酸酶测定，没有观察到对分化的影响，尽管SIT治疗7天后总酸性磷酸酶增加。结果表明，与SIT治疗相关的细胞数量减少和凋亡增加是通过激活鞘磷脂循环介导的。
Epidemiological evidence has shown that men consuming a low-fat, high-fiber diet containing high amounts of plant products have a lower risk of prostate cancer than men consuming a Western diet. One of the main differences between these two diets is the type of dietary fat, including dietary sterols. This study was undertaken to compare the effect of two dietary sterols on prostate cancer cells in vitro. beta-Sitosterol (SIT), the most common plant sterol, and cholesterol, an animal sterol, were compared for effect on LNCaP cell growth, differentiation, apoptosis, and sphingomyelin cycle intermediates. Cells were treated for up to seven days with sterols delivered by a cyclodextrin vehicle. Compared,vith cholesterol, SIT (16 mu M) decreased growth by 24% and induced apoptosis fourfold, which was accompanied by cell rounding and a 50% increase in ceramide production. No effect was observed on differentiation as measured by prostate-specific antigen and prostatic acid phosphatase, although total acid phosphatase increased with SIT treatment for up to seven days. The results suggest that the decrease in cell number and increase in apoptosis associated with SIT treatment are mediated by activating the sphingomyelin cycle.