YPEL5 protein of the YPEL gene family is involved in the cell cycle progression by interacting with two distinct proteins RanBPM and RanBP10
YPEL5 protein of the YPEL gene family is involved in the cell cycle progression by interacting with two distinct proteins RanBPM and RanBP10
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DOI：
10.1016/j.ygeno.2010.05.003
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发表时间：
2010-08-01
期刊：
影响因子：
4.4
通讯作者：
Minoshima, Shinsei
中科院分区：
文献类型：
--
作者：
Hosono, Katsuhiro;Noda, Setsuko;Minoshima, Shinsei
YPEL5是YPEL基因家族的一员，在真核物种中高度保守，显然参与某种与细胞分裂相关的功能。在这项研究中，我们更详细地研究了YPEL5蛋白的功能和系统发育方面。在细胞周期中，不同亚细胞定位检测到YPEL5蛋白；在间期，它位于细胞核和中心体中，在有丝分裂时，它依次改变位置到纺锤极、有丝分裂纺锤体和纺锤体中间区，最后在分裂时转移到体中部。通过siRNA或反义morpholino寡核苷酸敲低YPEL5功能可抑制培养的COS-7细胞的生长和medaka鱼胚胎的早期发育，表明其参与细胞周期进程。有趣的是，通过酵母双杂交方法鉴定出RanBPM（微管组织中心的Ran结合蛋白，由RANBP9编码）是一个ypel5结合蛋白。RanBPM的一个类似物，即RanBP10（由RanBP10编码），被发现是另一个ypel5结合蛋白，这两个蛋白基因彼此高度保守。比较基因组分析使我们能够定义一个由RanBPM和RanBP10组成的新基因家族，命名为Scorpin，为更好地理解它们如何与YPEL5相互作用提供了基础。(C) 2010爱思唯尔公司版权所有。
YPEL5 is a member of the YPEL gene family that is highly conserved in the eukaryotic species and apparently involved in a certain cell division-related function. In this study, we examined the functional and phylogenetic aspects of YPEL5 protein in more detail. During cell cycle, YPEL5 protein was detected at different subcellular localizations; at interphase, it was located in the nucleus and centrosome, then it changed location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferred to midbody at cytokinesis. Knockdown of YPEL5 function by siRNA or anti-sense morpholino oligonucleotide inhibited the growth of cultured COS-7 cells and early development of medaka fish embryos, indicating its involvement in cell cycle progression. Interestingly, RanBPM (Ran Binding Protein in the Microtubule organizing center, encoded by RANBP9) was identified as a YPEL5-binding protein by yeast two-hybrid method. A paralog of RanBPM, namely RanBP10 (encoded by RANBP10), was found to be another YPEL5-binding protein, and these two protein genes are highly conserved each other. Comparative genomic analysis allowed us to define a new gene family consisting of RanBPM and RanBP10, named Scorpin, providing a basis to better understand how they interact with YPEL5. (C) 2010 Elsevier Inc. All rights reserved.