EXPRESSION OF THE 2-5A SYSTEM DURING THE CELL-CYCLE
EXPRESSION OF THE 2-5A SYSTEM DURING THE CELL-CYCLE
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DOI：
10.1016/s0014-4827(85)80034-3
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发表时间：
1985-01-01
影响因子：
3.7
通讯作者：
MALLUCCI, L
中科院分区：
文献类型：
--
作者：
WELLS, V;MALLUCCI, L
为了确定2-5A系统是否在细胞生长调节中起作用，我们对小鼠胚胎成纤维细胞中2-5A通路的所有成分进行了检测，在第三阶段进行了1个周期的分裂，在每个周期阶段保持高度均匀的条件下。2-5A合成酶水平在S期晚期增加10倍，并随着细胞进入G2而下降。观察到2- 5a依赖性核糖核酸酶的类似但较小的增加，而2‘ 5’磷酸二酯酶的活性在静止细胞中最高。当合成酶水平达到最大值时，完整细胞中未检测到磷酸化的2-5A。在培养上清中发现内源性干扰素（IFN）浓度随着细胞周期的进展而增加，IRN抗体的加入减少了s晚期合成酶的增加。用细胞产生的生长抑制剂处理细胞也会影响合成酶的活性。
To determine whether the 2-5A system has a role in the regulation of cell growth all constituents of the 2-5A pathway in mouse embryo fibroblasts have been examined undergoing 1 cycle of division at the tertiary stage under conditions where a high degree of uniformity is maintained within each stage of the cycle. Levels of the 2-5A synthetase increased up to 10-fold late in S phase and declined as cells moved through G2. A similar but smaller increase in the 2-5A-dependent ribonuclease was observed, whereas activity of the 2''5'' phosphodiesterase was highest in quiescent cells. At the time of maximum synthetase levels no phosphorylated 2-5A could be detected in the intact cell. Endogenous interferon (IFN) was found in the culture supernatants in increasing concentration with cell cycle progression and addition of antibodies to IRN reduced the increase in synthetase seen in late S. Treatment of cells with a growth inhibitor that cells produce also affected synthetase activity.