On-line gas dialyzer for automated enzymatic analysis with potentiometric ammonia detection.
On-line gas dialyzer for automated enzymatic analysis with potentiometric ammonia detection.
复制标题
用于自动酶分析和电位氨检测的在线气体透析器。
DOI：
10.1021/ac00253a039
复制
发表时间：
1983
影响因子：
7.4
通讯作者：
Meyerhoff,ME
中科院分区：
文献类型：
--
作者：
Fraticelli,YM;Meyerhoff,ME
本文介绍了一种自动化酶分析系统，该系统具有氨电极检测功能，并与一种新型的在线酶分析装置相结合。预透析装置可在酶促步骤之前非破坏性地有效去除生理样品中正常和异常高背景水平的氨氮干扰物。该系统的临床应用通过使用可溶形式的酶L-天冬酰胺酶直接测量血浆样品中的L-天冬酰胺来证明。观察到的峰电位与样品底物浓度在数学上相关。当天冬酰胺浓度小于10~ 4 mol/L时，每小时至少可测定30个样品，精密度良好。本文介绍了有关系统变量如流速、稀释剂、透析管长度等的优化的基本研究。
An automated enzymatic assay system Incorporating ammo-nia electrode detection In conjunction with a novel on-line predlalysls unit is described. The predialysis unit nonde-structively and efficiently removes Interferent normal and abnormally high background levels of ammonia nitrogen from physiological samples prior to the enzymatic step. The clinical application of the system Is demonstrated via the direct measurement of L-asparagine In plasma samples through the use of the enzyme L-asparaginase In the soluble form. Peak potentials observed are logarithmically related to the sample substrate concentration. At least 30 samples/h can be as-sayed with excellent precision for asparagine concentrations< 10~ 4 mol/L. Fundamental Investigations concerning the optimization of the system variables such as flow rates, di-luent reagents, length of dialysis tubing, etc. are described.