THE AMINO-TERMINAL REGION OF AN IMPORTED MITOCHONDRIAL PRECURSOR POLYPEPTIDE CAN DIRECT CYTOPLASMIC DIHYDROFOLATE-REDUCTASE INTO THE MITOCHONDRIAL MATRIX
THE AMINO-TERMINAL REGION OF AN IMPORTED MITOCHONDRIAL PRECURSOR POLYPEPTIDE CAN DIRECT CYTOPLASMIC DIHYDROFOLATE-REDUCTASE INTO THE MITOCHONDRIAL MATRIX
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DOI：
10.1002/j.1460-2075.1984.tb02272.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
HURT, EC;PESOLDHURT, B;SCHATZ, G
酵母细胞色素c氧化酶亚基IV由核基因编码，在胞质溶胶中合成为具有25个氨基酸的瞬时氨基末端延伸的前体，并输入线粒体。基因融合将亚基IV前体的氨基末端53个氨基酸连接到小鼠胞质酶二氢叶酸还原酶的氨基末端。当所得融合蛋白在转录-翻译系统中合成，然后与通电的酵母线粒体一起孵育时，它被导入线粒体基质空间，并被螯合剂敏感的基质蛋白酶加工成较短的形式。没有证据表明融合蛋白被粘在2个线粒体膜之一上。如果非线粒体蛋白质装配有线粒体靶向序列，则其可以被转运到线粒体基质中。
Subunit IV of yeast cytochrome c oxidase is encoded by a nuclear gene, synthesized in the cytosol as a precursor with a transient amino-terminal extension of 25 amino acids, and imported into the mitochondria. Gene fusion attached the amino-terminal 53 amino acids of the subunit IV precursor to the amino terminus of the mouse cytosolic enzyme dihydrofolate reductase. When the resulting fusion protein was synthesized in a transcription-translation system and then incubated with energized yeast mitochondria, it was imported into the mitochondrial matrix space and processed to a shorter form by the chelator-sensitive matrix protease. No evidence was obtained that the fusion protein became stuck across one of the 2 mitochondrial membranes. A non-mitochondrial protein can be transported into the mitochondrial matrix if it is fitted with a mitochondrial targeting sequence.