A ROLE FOR MEMBRANE LIPID POLYUNSATURATION IN CHLOROPLAST BIOGENESIS AT LOW-TEMPERATURE
A ROLE FOR MEMBRANE LIPID POLYUNSATURATION IN CHLOROPLAST BIOGENESIS AT LOW-TEMPERATURE
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DOI：
10.1104/pp.99.1.197
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发表时间：
1992-05-01
期刊：
影响因子：
7.4
通讯作者：
SOMERVILLE, C
中科院分区：
文献类型：
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作者：
HUGLY, S;SOMERVILLE, C
两个缺乏叶绿体膜脂多不饱和的拟南芥突变体在22℃下生长时，外观上与野生型难以区分。相比之下，在5摄氏度的生长过程中发育的突变体的叶片组织是黄化的，而野生型不是。这是叶绿体脂质多不饱和有助于低温适应性的第一个直接证据。在5℃下生长的突变体的叶绿体比野生型小得多，类囊体膜含量降低了70%。然而，低温对暴露于低温之前形成的叶绿体没有明显的影响。这些和相关的观察表明，叶绿体生物发生的某些方面需要高度的叶绿体膜脂多不饱和度。
Two different mutants of Arabidopsis thaliana deficient in chloroplast membrane lipid polyunsaturation were indistinguishable in appearance from the wild-type when grown at 22-degrees-C. By contrast, leaf tissues of the mutants that developed during growth at 5-degrees-C were chlorotic, whereas the wild type was not. This is the first direct evidence that chloroplast lipid polyunsaturation contributes to low-temperature fitness. Chloroplasts from mutant lines grown at 5-degrees-C were much smaller than those of the wild-type, and the thylakoid membrane content was reduced by up to 70%. However, there was no discernible effect of low temperature on chloroplasts that developed prior to exposure to low temperatures. These and related observations suggest that the high degree of chloroplast membrane lipid polyunsaturation is required for some aspect of chloroplast biogenesis.