Vitamin D3 pretreatment protects against lipopolysaccharide-induced earlyembryo loss through its anti-inflammatory effects
Vitamin D3 pretreatment protects against lipopolysaccharide-induced earlyembryo loss through its anti-inflammatory effects
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维生素 D3 预处理通过其抗炎作用防止脂多糖诱导的早期胚胎丢失
DOI：
10.1111/aji.12620
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Xu De-Xiang
中科院分区：
文献类型：
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作者：
Zhou Yan;Chen Yuan-Hua;Fu Lin;Yu Zhen;Xia Mi-Zhen;Hu Xiao-Guang;Wang Hua;Xu De-Xiang
越来越多的证据表明，炎症细胞因子参与LPS诱导的不良妊娠结局，包括早期胚胎丢失。维生素D3（VitD 3）具有抗炎活性。我们旨在研究维生素D3（VitD 3）对LPS诱导的小鼠早期胚胎丢失的影响。研究方法除对照组外，所有妊娠小鼠在GD 7腹腔内（ip）注射LPS。单独VitD 3组和LPS+ VitD 3组于妊娠第5天至第7天每天灌胃VitD 3。有趣的是，当妊娠小鼠用VitD 3预处理时，流产率下降到14.3%。另外的实验显示，VitD 3显著减弱LPS诱发的母体血清中TNF-α、IFN-γ、MIP-2和硝酸盐+亚硝酸盐的升高。此外，VitD 3可减轻LPS诱导的蜕膜考克斯-2表达，并减弱母体血清中PGF 2 α的升高。虽然VitD 3对母体血清中的IL-10没有影响，但它在LPS处理的小鼠中诱导了血清IL-10水平的进一步升高。结论VitD 3对LPS诱导的小鼠早期胚胎丢失具有保护作用，其机制可能与其抗炎作用有关。
ProblemIncreasing evidence demonstrates that inflammatory cytokines are involved in LPS‐induced adverse pregnant outcomes including early embryo loss. Vitamin D3 (VitD3) has anti‐inflammatory activity. We aimed to investigate the effects of vitamin D3 (VitD3) on LPS‐induced early embryo loss in mice.Method of studyAll pregnant mice except controls were intraperitoneally (ip) injected with LPS on GD7. In VitD3 alone and LPS+VitD3 groups, pregnant mice were pretreated with VitD3 by gavage daily from GD5 to GD7.ResultsLPS caused 62.5% pregnant mice with early embryo loss. Interestingly, the rate of abortion dropped to 14.3% when pregnant mice were pretreated with VitD3. Additional experiment showed that VitD3 significantly attenuated LPS‐evoked elevation on TNF‐α, IFN‐γ, MIP‐2, and nitrate plus nitrite in maternal serum. In addition, VitD3 alleviated LPS‐induced COX‐2 expression in the decidua and attenuated the elevation of PGF2α in maternal serum. Although VitD3 had no effect on IL‐10 in maternal serum, it induced further elevation of serum IL‐10 level in LPS‐treated mice. Further analysis showed that VitD3 activated VDR signaling, simultaneously inhibited LPS‐induced nuclear translocation of NF‐κB p65 subunits in the decidua.ConclusionsVitD3 protects mice from LPS‐induced early embryo loss at least partially through its anti‐inflammatory effects.