INTRAARTICULAR INJECTIONS WITH METHYLPREDNISOLONE ACETATE REDUCE OSTEOARTHRITIC LESIONS IN PARALLEL WITH CHONDROCYTE STROMELYSIN SYNTHESIS IN EXPERIMENTAL OSTEOARTHRITIS
INTRAARTICULAR INJECTIONS WITH METHYLPREDNISOLONE ACETATE REDUCE OSTEOARTHRITIC LESIONS IN PARALLEL WITH CHONDROCYTE STROMELYSIN SYNTHESIS IN EXPERIMENTAL OSTEOARTHRITIS
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DOI：
10.1002/art.1780370316
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发表时间：
1994-03-01
影响因子：
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通讯作者：
MARTELPELLETIER, J
中科院分区：
文献类型：
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作者：
PELLETIER, JP;MINEAU, F;MARTELPELLETIER, J
目标。目的：观察关节腔内注射甲基强的松龙(MA)对实验性骨关节炎(OA)骨关节病变和软骨细胞基质分解素合成的影响。15只杂种犬右膝前十字韧带被刺伤处切断。8只狗在手术时和4周后接受了MA(20 Mg)关节内注射；7只没有接受治疗。这些狗在手术后8周被处死。另取5只正常犬作为对照。对损伤进行了宏观评估，包括测量骨赘和髁突和平台上的表面损伤面积，并对损伤的严重程度进行了组织学评估。用兔抗基质分解素多克隆抗体进行免疫组织化学分析，然后用形态计量学分析评价基质和软骨细胞染色。MA治疗显著降低了骨赘的发生率(P<；0.0004)和大小(P<；0.0001)。软骨损伤的组织学分级也显著降低(P<；0.01)和平台(P<；0.002)。免疫组织化学研究显示，对于OA软骨，基质分解素阳性的软骨细胞百分比显著增加(P<；0.002)，整个软骨各层的染色强度以及特殊的基质染色显著增加(P<；0.005)。MA处理使软骨细胞(P<；0.002.0 1)和基质(P<；0.0 1)的染色水平接近正常。这些发现为皮质类固醇注射对OA损伤的保护作用提供了额外的证据，并表明该药物的作用可能是通过抑制基质分解素的合成来实现的。
Objective. To examine the effect of intraarticular injections of methylprednisolone acetate (MA) on osteoarthritic lesions and chondrocyte stromelysin synthesis in experimental osteoarthritis (OA).Methods. In 15 mongrel dogs, the anterior cruciate ligament of the right knee was sectioned by a stab wound. Eight dogs received intraarticular injections of MA (20 mg) at the time of surgery and 4 weeks later; 7 had no treatment. The dogs were killed 8 weeks after surgery. Five normal dogs were used as controls. Macroscopic evaluation of the lesions, including measurements of osteophytes and areas of surface lesions on the condyles and plateaus, was conducted, along with histologic evaluation of the severity of lesions. Immunohistochemical analysis was carried out using a rabbit polyclonal antibody against stromelysin, followed by evaluation of matrix and chondrocyte staining using morphometric analysis.Results. Treatment with MA significantly reduced the incidence (P < 0.0004) and size (P < 0.0001) of osteophytes. The histologic grading of cartilage lesions was also significantly reduced both on condyles (P < 0.01) and on plateaus (P < 0.002). Immunohistochemical studies revealed, for OA cartilage, a marked increase (P < 0.002) in the percentage of chondrocytes positive for stromelysin and in the intensity of staining throughout all the layers of the cartilage, as well as specific matrix staining (P < 0.005). Treatment with MA reduced staining at both the chondrocyte (P < 0.002) and the matrix (P < 0.01) levels toward normal.Conclusion. These findings provide additional evidence for the protective effect of corticosteroid injections on OA lesions, and indicate that the effect of this drug may be mediated through the suppression of stromelysin synthesis.