Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody
Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody
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DOI：
10.1111/j.1440-1827.2008.02214.x
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发表时间：
2008-04-01
影响因子：
2.2
通讯作者：
Moriyama, Masatsugu
中科院分区：
文献类型：
--
作者：
Kanada, Ryoko;Uchida, Tomohisa;Moriyama, Masatsugu
据报道，在胃癌死亡率较高的东亚国家中流行的幽门螺杆菌细胞毒素相关抗原A（CagA）在结构上与胃癌死亡率相对较低的西方国家不同。根据位于蛋白质羧基末端的EPIYA基序的结构特征，CagA被细分为两种类型：东亚CagA和西方CagA。最近的一项研究表明，使用抗东亚特异性抗体（α-EAS Ab）进行免疫组织化学分析可能有助于诊断 cagA 基因型，该抗体与东亚 CagA 发生特异性免疫反应，但与西方 CagA 不发生反应。为了进一步评估该诊断方法在敏感性、特异性和准确性方面的价值，对 143 份含有 α-EAS Ab 的胃活检标本进行了免疫组织化学分析，并与 cagA 基因测序进行了比较。结果发现，使用α-EAS Ab进行免疫组化诊断幽门螺杆菌cagA基因型具有高度敏感性（敏感性93.2%）和特异性（特异性72.7%），表明cagA基因型的免疫组化诊断对于cagA基因型的诊断是有用的。
The cytotoxin-associated antigen A (CagA) of Helicobacter pylori prevalent in East Asian countries, where the mortality rate due to gastric cancer is high, has been reported to be structurally different from that in Western countries, where the gastric cancer mortality rate is relatively low. Based on the structural features of the EPIYA motifs located at the carboxyl terminal of the protein, CagA was subdivided into two types: East Asian CagA and Western CagA. A recent study suggested that immunohistochemistry with anti-East Asian-specific antibody (alpha-EAS Ab), which was specifically immunoreactive with East Asian CagA but not with Western CagA, may be useful for diagnosis of the cagA genotype. To further evaluate the value of this diagnostic method in terms of sensitivity, specificity, and accuracy, 143 gastric biopsy specimens with alpha-EAS Ab were analyzed on immunohistochemistry and compared with the sequencing of the cagA gene. It was found that diagnosis of the cagA genotype of H. pylori on immunohistochemistry using the alpha-EAS Ab was highly sensitive (sensitivity 93.2%) and specific (specificity 72.7%), suggesting that immunohistochemical diagnosis of the cagA genotype is useful for diagnosis of the cagA genotype.