Organotypic stromal cells impact endothelial cell transcriptome in 3D microvessel networks.
Organotypic stromal cells impact endothelial cell transcriptome in 3D microvessel networks.
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DOI：
10.1038/s41598-022-24013-y
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发表时间：
2022-11-28
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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Endothelial cells line all major blood vessels and serve as integral regulators of many functions including vessel diameter, cellular trafficking, and transport of soluble mediators. Despite similar functions, the phenotype of endothelial cells is highly organ-specific, yet our understanding of the mechanisms leading to organ-level differentiation is incomplete. We generated 3D microvessel networks by combining a common naïve endothelial cell with six different stromal cells derived from the lung, skin, heart, bone marrow, pancreas, and pancreatic cancer. Single cell RNA-Seq analysis of the microvessel networks reveals five distinct endothelial cell populations, for which the relative proportion depends on the stromal cell population. Morphologic features of the organotypic vessel networks inversely correlate with a cluster of endothelial cells associated with protein synthesis. The organotypic stromal cells were each characterized by a unique subpopulation of cells dedicated to extracellular matrix organization and assembly. Finally, compared to cells in 2D monolayer, the endothelial cell transcriptome from the 3D in vitro heart, skin, lung, and pancreas microvessel networks are more similar to the in vivo endothelial cells from the respective organs. We conclude that stromal cells contribute to endothelial cell and microvessel network organ tropism, and create an endothelial cell phenotype that more closely resembles that present in vivo.
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DOI：
10.1039/c3ib40170g
发表时间：
2014-06
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Ehsan SM;Welch-Reardon KM;Waterman ML;Hughes CC;George SC
通讯作者：
George SC
影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
DOI：
10.1073/pnas.97.1.85
发表时间：
2000-01-04
影响因子：
11.1
作者：
Brown, JH;Volkmann, N;Cohen, C
通讯作者：
Cohen, C
影响因子：
4.6
作者：
Auvinen, Kaisa;Lokka, Emmi;Salmi, Marko
通讯作者：
Salmi, Marko
缺乏周细胞会导致内皮增生和异常血管形态发生。
影响因子：
7.8
作者：
Hellstrom, M;Gerhardt, H;Kalen, M;Li, X;Eriksson, U;Wolburg, H;Betsholtz, C
通讯作者：
Betsholtz, C