Establishing microbial co‐cultures for 3‐hydroxybenzoic acid biosynthesis on glycerol
Establishing microbial co‐cultures for 3‐hydroxybenzoic acid biosynthesis on glycerol
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DOI：
10.1002/elsc.201800195
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发表时间：
2019-05
影响因子：
2.7
通讯作者：
Yiyao Zhou;Zhenghong Li;Xiaonan Wang;Haoran Zhang
中科院分区：
文献类型：
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作者：
Yiyao Zhou;Zhenghong Li;Xiaonan Wang;Haoran Zhang
使用工程微生物将可再生原料转化为芳香族化合物，为可持续，环境友好和具有成本效益的生产这些增值产品提供了一种强大的方法，而无需依赖石油。本研究通过合理设计E.大肠杆菌大肠杆菌共培养系统建立用于将甘油转化为3-羟基苯甲酸（3 HB）。具体而言，3 HB途径被两个代谢工程化的E.大肠杆菌菌株。通过使用不同的培养温度、改变共培养菌株之间的接种比例、募集关键途径中间转运蛋白、加强关键途径酶表达和调整诱导途径基因表达的时机，优化共培养生物合成。与E. coli单培养物中，优化的共培养物显示3 HB生物合成提高了5.3倍。这项研究证明了模块化共培养工程在解决芳香族化合物生物合成挑战方面的适用性。
Converting renewable feedstocks to aromatic compounds using engineered microbes offers a robust approach for sustainable, environment‐friendly, and cost‐effective production of these value‐added products without the reliance on petroleum. In this study, rationally designed E. coli–E. coli co‐culture systems were established for converting glycerol to 3‐hydroxybenzoic acid (3HB). Specifically, the 3HB pathway was modularized and accommodated by two metabolically engineered E. coli strains. The co‐culture biosynthesis was optimized by using different cultivation temperatures, varying the inoculum ratio between the co‐culture strains, recruitment of a key pathway intermediate transporter, strengthening the critical pathway enzyme expression, and adjusting the timing for inducing pathway gene expression. Compared with the E. coli mono‐culture, the optimized co‐culture showed 5.3‐fold improvement for 3HB biosynthesis. This study demonstrated the applicability of modular co‐culture engineering for addressing the challenges of aromatic compound biosynthesis.