Effect of direct injection of melanin-concentrating hormone into the paraventricular nucleus: Further evidence for a stimulatory role in the adrenal axis via SLC-1
Effect of direct injection of melanin-concentrating hormone into the paraventricular nucleus: Further evidence for a stimulatory role in the adrenal axis via SLC-1
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DOI：
10.1046/j.1365-2826.2003.00997.x
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发表时间：
2003-03-01
影响因子：
3.2
通讯作者：
Bloom, SR
中科院分区：
文献类型：
--
作者：
Kennedy, AR;Todd, JF;Bloom, SR
黑色素浓缩激素（MCH）与包括下丘脑-垂体-肾上腺（HPA）轴在内的许多激素轴的控制有关。先前的研究表明，有证据表明对下丘脑轴有刺激和抑制作用；因此，我们试图进一步表征MCH对该轴的影响。脑室内注射MCH可增加注射后10分钟循环促肾上腺皮质激素（ACTH）。注射MCH直接进入室旁核（PVN）后10 min，循环ACTH和皮质酮均升高。此外，MCH还能增加下丘脑外植体促肾上腺皮质激素释放因子（CRF）的释放，而这种作用被SLC-1特异性拮抗剂SB-568849所消除。神经肽EI，一种与MCH来自相同前体的肽，也被发现可以增加外植体的CRF释放。这些结果表明MCH通过SLC-1对HPA轴具有刺激作用，并且MCH主要通过PVN CRF神经元群发挥其作用。
Melanin-concentrating hormone (MCH) is implicated in the control of a number of hormonal axes including the hypothalamic-pituitary adrenal (HPA) axis. Previous studies have shown that there is evidence for both a stimulatory and an inhibitory action on the HPA axis; therefore, we attempted to further characterize the effects of MCH on this axis. Intracerebroventricular injection of MCH increased circulating adrenocorticotropic hormone (ACTH) at 10 min post injection. Injection of MCH directly into the paraventricular nucleus (PVN) was found to increase both circulating ACTH and corticosterone 10 min after injection. Additionally, MCH was found to increase corticotropin-releasing factor (CRF) release from hypothalamic explants, and this effect was abolished by the specific SLC-1 antagonist SB-568849. Neuropeptide EI, a peptide from the same precursor as MCH was also found to increase CRF release from explants. These results suggest that MCH has a stimulatory role in the HPA axis via SLC-1, and that MCH exerts its effects predominantly through the PVN CRF neuronal populations.