Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma.
Critical role for the long non-coding RNA AFAP1-AS1 in the proliferation and metastasis of hepatocellular carcinoma.
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DOI：
10.1007/s13277-016-4858-8
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Zhang S
中科院分区：
文献类型：
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作者：
Lu X;Zhou C;Li R;Liang Z;Zhai W;Zhao L;Zhang S
越来越多的证据表明，长链非编码 RNA (lncRNA) 的失调可能导致人类癌症（包括 HCC）的进展和转移。此前的研究表明，lncRNA AFAP1-AS1 在癌症中发挥着关键作用。然而，AFAP1-AS1 在 HCC 中的作用仍有待确定。在本研究中，发现AFAP1-AS1在HCC组织中增加，并且AFAP1-AS1的高表达与肿瘤大小、TNM分期、血管侵犯和不良预后相关。体外实验中，沉默 AFAP1-AS1 可显着降低细胞增殖、克隆生长、细胞迁移和侵袭，并增加细胞凋亡。此外，在小鼠体内同种异体移植模型中，AFAP1-AS1 沉默显着减少了肿瘤生长。结果表明AFAP1-AS1在HCC的发展中很重要，并且可以作为HCC的治疗靶点。
Increasing evidence has indicated that dysregulation of long non-coding RNAs (lncRNAs) can contribute to the progression and metastasis of human cancer, including HCC. Previous studies have shown that the lncRNA AFAP1-AS1 plays a critical role in cancer. However, the roles of AFAP1-AS1 in HCC remain to be determined. In the present study, AFAP1-AS1 was found to be increased in HCC tissues, and high AFAP1-AS1 expression was associated with tumor size, TNM stage, vascular invasion, and poor prognosis. Silencing of AFAP1-AS1 significantly reduced cell proliferation, clonal growth, cell migration, and invasion and increased apoptosis in vitro. Furthermore, AFAP1-AS1 silencing markedly reduced tumor growth in a murine allograft model in vivo. The results suggested that AFAP1-AS1 is important in HCC development and serves as a therapeutic target of HCC.