The destruction evaluation in different foot joints: new ideas in collagen-induced arthritis rat model
The destruction evaluation in different foot joints: new ideas in collagen-induced arthritis rat model
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DOI：
10.1007/s00296-008-0731-4
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发表时间：
2009-04
影响因子：
4
通讯作者：
Peng Zhang;Dong Han;Ting‐ting Tang;Xiaoling Zhang;K. Dai
中科院分区：
文献类型：
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作者：
Peng Zhang;Dong Han;Ting‐ting Tang;Xiaoling Zhang;K. Dai
自1977年以来，胶原诱导性关节炎（CIA）已被广泛用作类风湿关节炎的动物模型，但迄今为止，还没有文献描述不同足关节的破坏特征。本研究通过观察CIA足关节破坏过程的差异，进一步阐明CIA足关节破坏的病理过程。用牛Ⅱ型胶原和弗氏不完全佐剂免疫雄性Wistar大鼠，诱导CIA。在第二次免疫后1、2、4、6和8个月进行放射学研究，以跟踪疾病的发展。最后处死动物进行组织学观察。在组织学观察中，关节破坏主要有三种类型：软骨下侧侵蚀、关节外侵蚀和关节软骨的软骨融合。所有这些破坏形式都存在于一个或多个不同的关节中。此外，我们发现抗酒石酸酸性磷酸酶（TRAP）染色阳性细胞参与了关节软骨的破坏。这些新发现表明CIA模型在发病过程中，不同足关节表现出不同的破坏特征，足关节软骨融合是另一个典型的病理特征。
Collagen-induced arthritis (CIA) has been widely used as the animal model of rheumatoid arthritis since 1977, while till now, no paper has depicted the destruction characteristics in different foot joints. In this study, we observed the differences among the foot joint destruction process of CIA to elucidate further the pathological process of this model. CIA was induced in male Wistar rat immunized with bovine type II collagen and Freund’s incomplete adjuvant. Radiological studies were performed 1, 2, 4, 6, and 8 months after the second immunization to follow the development of disease. At last, all the animals were killed and histological research was performed. In the histological observation, three main types of joint destructions such as subchondral side erosion, external joint erosion and the cartilaginous fusion of articular cartilage were identified. All these destruction forms exist in one joint or several different joints. Furthermore, we found that tartrate-resistant acid phosphatase (TRAP) stain-positive cells participated in the destruction of articular cartilage. These new findings showed that in the disease process of the CIA model, different foot joints show different destruction characteristics and cartilaginous fusion of foot joints is another typical pathological characteristic.