The bioavailability of ferulic acid is governed primarily by the food matrix rather than its metabolism in intestine and liver in rats
The bioavailability of ferulic acid is governed primarily by the food matrix rather than its metabolism in intestine and liver in rats
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DOI：
10.1093/jn/132.7.1962
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发表时间：
2002-07-01
影响因子：
4.2
通讯作者：
Rémésy, C
中科院分区：
文献类型：
--
作者：
Adam, A;Crespy, V;Rémésy, C
阿魏酸（FA）的生理重要性，特别是其抗氧化特性，取决于其吸收和随后与靶组织相互作用的可用性。由于FA广泛存在于谷物中，本研究的目的是通过原位肠灌注模型（10 - 50 nmol/min）研究其在大鼠肠道和肝脏中的代谢，以及其在补充饮食（10 - 250 mumol/d）或复杂谷物基质（即，来自Valoris（Triticum aestivum）或Duriac（T. durum）栽培品种和来自Valoris栽培品种的麸皮或白色面粉。在灌流大鼠肠道中，FA的净吸收与灌流剂量成比例（R-2 = 0.997）;一旦吸收，FA在血浆和胆汁分泌中以结合形式完全回收（占灌流剂量的5-7%）。在喂食富含FA的半纯化饲料的大鼠中，FA的吸收非常有效，因为近似50%的摄入量在尿液中回收。这种通过肾脏的广泛消除限制了FA在血浆中的蓄积（在喂食50 mumol FA/d的大鼠中通常为1 mumol/L）。相反，在喂食56-81 mumolFA/d谷物饲料的大鼠中，尿排泄量比喂食富含FA的半纯化饲料的大鼠低90-96%，血浆浓度与0.2 -0.3 mumol/L相似。因此，谷物基质似乎严重限制了FA的生物利用度。谷物中FA的这种固有的低生物利用度可能反映了FA通过与阿拉伯聚糖和木质素交联而与纤维部分的关联。
The physiologic importance of ferulic acid (FA), and notably its antioxidant properties, depends upon its availability for absorption and subsequent interaction with target tissues. Because FA is widely present in cereals, the aim of the present study was to investigate its intestinal and hepatic metabolism in rats by in situ intestinal perfusion model (from 10 to 50 nmol/min), and its bioavailability in supplemented diets (from 10 to 250 mumol/d) or in a complex cereal matrix, i.e., whole flours from Valoris (Triticum aestivum) or Duriac (T. durum) cultivars and bran or white flour from the Valoris cultivar. In perfused rat intestine, net FA absorption was proportional to the per-fused dose (R-2 = 0.997); once absorbed, FA was completely recovered as conjugated forms in plasma and bile secretion (representing 5-7% of the perfused dose). In rats fed FA-enriched semipurified diets, FA absorption was quite efficient because similar to50% of the ingested dose was recovered in urine. This extensive elimination by kidneys limited FA accumulation in plasma (typically 1 mumol/L in rats fed 50 mumol FA/d). In contrast, in rats fed cereal diets providing 56-81 mumol FA/d, urine excretion was 90-96% lower than in rats fed FA-enriched semipurified diets, and plasma concentrations were similar to0.2-0.3 mumol/L. Thus, the cereal matrix appears to severely limit FA bioavailability. This inherently low bioavailability of FA in cereals likely reflects FA association with the fiber fraction through cross-linking with arabinoxylans and lignins.