Correlations of Cell Surface Antigens with Individual Differences in Radiosensitivity in Human Hematopoietic Stem/Progenitor Cells
Correlations of Cell Surface Antigens with Individual Differences in Radiosensitivity in Human Hematopoietic Stem/Progenitor Cells
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DOI：
10.1667/rr1839.1
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发表时间：
2010-02-01
影响因子：
3.4
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
--
作者：
Takahashi, Kenji;Monzen, Satoru;Kashiwakura, Ikuo
为了研究不同群体的人造血干/祖细胞(HSPC)辐射敏感性的差异，我们研究了细胞表面抗原、克隆形成能力和辐射存活之间的关系。对20例胎盘/脐带血标本中CD34抗原表达丰富的HSPC表面CD34、CD38、CD45RA、CD110和Tie-2等早期分化途径相关抗原的表达进行了分析。CD38抗原与CD45RA、CD110与Tie-2呈显著正相关。在大多数病例中，抗原与集落形成细胞(CFCs)的数量之间没有明显的关系，但巨核祖细胞的数量与Tie-2(+)细胞的百分比呈负相关。Tie-2细胞百分率与2GyX射线照射后CFCs的存活率显著相关，提示单个CFCs群体的辐射敏感性与Tie-2表达细胞百分率有关。此外，祖细胞数量与2GyX射线照射后的存活分数密切相关。这些结果表明，单个HSPC群体的辐射敏感性与群体中祖细胞的数量有关，特别是依赖于未成熟HSPC的存在，如Tie-2(+)细胞。(C)2010年，由辐射研究学会提供
To characterize the differences in the radiosensitivity of individual populations of human hematopoietic stem/progenitor cells (HSPCs), we examined the relationship among cell surface antigens, clonogenic potential and radiation survival. The expressions of CD34, CD38, CD45RA, CD110 and Tie-2, early differentiation pathway-related antigens in hematopoiesis, were analyzed on the surface of HSPCs enriched for CD34 antigen expression in 20 samples prepared from human placental/umbilical cord blood. A significantly positive relationship was observed between CD38 antigen and CD45RA and between CD110 and Tie-2. No significant relationship was observed in most cases among the antigens and the number of colony-forming cells (CFCs); however, the number of megakaryocytic progenitor cells correlated negatively with the percentage of Tie-2(+) cells. The percentage Tie-2 cells correlated significantly with the surviving fraction of CFCs irradiated with 2 Gy of X rays, suggesting that the radiosensitivity of individual CFC populations is related to the percentage of Tie-2-expressing cells. In addition, the number of progenitor cells closely correlated with the surviving fraction after 2 Gy of X rays. These results suggest that the radiosensitivity of individual HSPC populations is related to the number of progenitor cells in the population especially dependent on the presence of immature HSPCs such as Tie-2(+) cells. (C) 2010 by Radiation Research Society