Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure
Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure
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DOI：
10.1111/1440-1681.12130
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发表时间：
2013-09-01
影响因子：
2.9
通讯作者：
Kohzuki, Masahiro
中科院分区：
文献类型：
--
作者：
Ito, Daisuke;Ito, Osamu;Kohzuki, Masahiro
1. 心脏和肾脏疾病之间存在相互作用，这种疾病称为心肾综合征。运动训练具有心脏保护作用，涉及心血管系统中内皮 (e) 一氧化氮合酶 (NOS) 的上调。然而，运动训练对心脏病患者肾脏中 NOS 的影响尚不清楚。 2.本研究的目的是探讨运动训练是否上调慢性心力衰竭（CHF）大鼠肾脏、左心室和主动脉中的NOS。 3.雄性Sprague-Dawley大鼠接受左冠状动脉结扎(LCAL)以诱导CHF，并在LCAL后4周被随机分配到静坐组或跑步机运动组。运动4周后三天，收集24小时的尿样，并在断头后收集血样。检查一氧化氮合酶活性和蛋白质表达。 4.在心肾功能参数上观察到CHF与运动训练之间存在显着的交互作用。运动训练可改善 CHF 大鼠的心功能、降低血浆 B 型利钠肽水平、减少尿白蛋白排泄并增加肌酐清除率。 CHF大鼠左心室和肾脏中一氧化氮合酶活性、eNOS表达和神经元（n）NOS表达显着降低。运动训练显着增加 NOS 活性以及 eNOS 和 nNOS 表达。 5. 肾脏和左心室中 NOS 的上调可能部分有助于运动训练对 CHF 大鼠心肾综合征的肾脏和心脏保护作用。
1. There is an interaction between heart and kidney diseases, which is a condition termed cardiorenal syndrome. Exercise training has cardioprotective effects, involving upregulation of endothelial (e) nitric oxide synthase (NOS) in the cardiovascular system. However, the effects of exercise training on NOS in the kidney with heart disease are unknown. 2. The aim of the present study was to investigate whether exercise training upregulates NOS in the kidney, left ventricle and aorta of rats with chronic heart failure (CHF). 3. Male Sprague-Dawley rats underwent left coronary artery ligation (LCAL) to induce CHF and were randomly assigned to sedentary or treadmill exercise groups 4 weeks after LCAL. Three days after exercising for 4 weeks, urine samples were collected for 24 h and blood samples were collected following decapitation. Nitric oxide synthase activity and protein expression were examined. 4. Significant interactions between CHF and exercise training were observed on parameters of cardiac and renal function. Exercise training improved cardiac function, decreased plasma B-type natriuretic peptide levels, decreased urinary albumin excretion and increased creatinine clearance in CHF rats. Nitric oxide synthase activity, eNOS expression and neuronal (n) NOS expression were significantly decreased in the left ventricle and kidney of CHF rats. Exercise training significantly increased NOS activity and eNOS and nNOS expression. 5. Upregulation of NOS in the kidney and left ventricle may contribute, in part, to the renal and cardiac protective effects of exercise training in cardiorenal syndrome in CHF rats.