Evaluation of intraretinal migration of retinal pigment epithelial cells in age-related macular degeneration using polarimetric imaging.
Evaluation of intraretinal migration of retinal pigment epithelial cells in age-related macular degeneration using polarimetric imaging.
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DOI：
10.1038/s41598-017-03529-8
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发表时间：
2017-06-09
影响因子：
4.6
通讯作者：
Goto H
中科院分区：
文献类型：
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作者：
Miura M;Makita S;Sugiyama S;Hong YJ;Yasuno Y;Elsner AE;Tamiya S;Tsukahara R;Iwasaki T;Goto H
本研究的目的是利用旋光法评价年龄相关性黄斑变性（AMD）视网膜色素上皮（RPE）细胞的视网膜内迁移。我们评估了155只处于不同AMD阶段的眼睛。使用偏振敏感扫描激光检眼镜（PS-SLO）计算去偏振光图像，并使用偏振敏感光学相干断层扫描（OCT）计算偏振均匀度。将每个偏振图像与488 nm（SW-AF）和787 nm（NIR-AF）处的相应自体荧光（AF）图像进行比较。视网膜内RPE迁移通过PS-SLO和PS-OCT图像上视网膜内高反射灶处存在的去极化以及NIR-AF和SW-AF图像上存在的高AF来定义。52/155眼（33.5%）的患者发生了RPE移位，其中玻璃状色素上皮脱离（PED）和浆液性PED的发生率明显高于其他组（P = 0.015）。浆液性PED中迁移的RPE簇的体积与PED的体积显著相关（R2 = 0.26; P = 0.011）。总的来说，我们的研究结果表明，视网膜内RPE迁移发生在不同的AMD阶段，他们更常见于浆液性和玻璃疣样PED的眼睛。
The purpose of the present study was to evaluate the intraretinal migration of the retinal pigment epithelium (RPE) cells in age-related macular degeneration (AMD) using polarimetry. We evaluated 155 eyes at various AMD stages. Depolarized light images were computed using a polarization-sensitive scanning laser ophthalmoscope (PS-SLO), and the degree of polarization uniformity was calculated using polarization-sensitive optical coherence tomography (OCT). Each polarimetry image was compared with the corresponding autofluorescence (AF) images at 488 nm (SW-AF) and at 787 nm (NIR-AF). Intraretinal RPE migration was defined by the presence of depolarization at intraretinal hyperreflective foci on PS-SLO and PS-OCT images, and by the presence of hyper-AF on both NIR-AF and SW-AF images. RPE migration was detected in 52 of 155 eyes (33.5%) and was observed in drusenoid pigment epithelial detachment (PED) and serous PED with significantly higher frequencies than in other groups (P = 0.015). The volume of the migrated RPE cluster in serous PED was significantly correlated with the volume of the PED (R2 = 0.26; P = 0.011). Overall, our results showed that intraretinal RPE migrations occurred in various AMD stages, and that they occurred more commonly in eyes with serous and drusenoid PED.