Cecal Ligation and Puncture
Cecal Ligation and Puncture
复制标题
DOI：
10.1007/978-1-0716-1488-4_1
复制
发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Osuchowski, Marcin
中科院分区：
文献类型：
--
作者：
Drechsler, Susanne;Osuchowski, Marcin
盲肠结扎和穿孔（CLP）被称为腹部脓毒症的“金标准”啮齿动物模型。CLP在腹部产生持续泄漏的多微生物感染病灶。在全身麻醉和镇痛下打开腹腔，暴露盲肠，在回盲瓣下结扎，并用针刺穿。少量粪便通过穿孔压出，盲肠重新定位到腹部，然后用单纽扣缝合线和组织胶闭合腹部。CLP的严重程度可以通过结扎盲肠的长度以及针的大小来影响。在24小时内，动物出现全身性细菌感染的临床体征。在脓毒症急性期应给予镇痛、广泛的抗生素治疗和液体复苏，以增加CLP模型的临床相关性。
Cecal ligation and puncture (CLP) is referred to as the "gold standard" rodent model for abdominal sepsis. CLP creates a continuously leaking, polymicrobial infectious focus in the abdomen. The abdominal cavity is opened under general anesthesia and analgesia and the cecum is exposed, ligated underneath the ileocecal valve, and punctured with a needle. A small amount of feces is pressed out through the puncture and the cecum is repositioned into the abdomen, which is then closed with single button sutures and tissue glue. CLP severity can be influenced via the length of the ligated cecum as well as the needle size. Within 24 h, animals develop clinical signs of a systemic bacterial infection. Analgesia, wide range antibiotic treatment, and fluid resuscitation should be administered during the acute phase of sepsis to increase the clinical relevance of the CLP model.