Progesterone and estrogen secretion by cultured monkey ovarian cell types: influences of follicular size, serum luteinizing hormone levels, and follicular fluid estrogen levels.
Progesterone and estrogen secretion by cultured monkey ovarian cell types: influences of follicular size, serum luteinizing hormone levels, and follicular fluid estrogen levels.
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培养的猴卵巢细胞类型的黄体酮和雌激素分泌：卵泡大小、血清黄体生成激素水平和卵泡液雌激素水平的影响。
DOI：
10.1210/endo-107-1-342
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发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
C. Channing
中科院分区：
文献类型：
--
作者：
C. Channing
在促黄体生成素高峰前和促黄体生成素高峰期剖腹，取出卵泡层、间质和颗粒细胞，分别或联合培养8d，加入或不加入5×10-7M睾酮。在每个实验中(n=30)，在卵泡采集前立即测定卵泡液和血清中的雌激素和孕酮。卵母细胞取自最大的(推测为排卵前)卵泡；用立体显微镜测定其成熟度，然后将卵母细胞培养2天。含有不能附着和生长的颗粒细胞或退化的卵母细胞(在收获时或在培养中退化)的卵泡不被使用，并被指定为闭锁。在黄体生成素高峰开始后，卵泡液中孕酮和雌激素水平显著升高，雌激素水平在黄体生成素高峰时达到峰值。促黄体生成素高峰后第一天，卵泡液和血清雌激素水平下降。
Rhesus monkeys were laparotomized before and during the LH surge, and the thecal layer, stroma, and granulosa cells were removed and cultured for 8 days, separately or together, in the presence or absence of 5 × 10-7 M testosterone. In each experiment (n = 30), estrogen and progesterone were measured in the follicular fluid and serum immediately before follicular harvest. The oocyte was harvested from the largest (presumed preovulatory) follicle present; its degree of maturity was determined using a stereomicroscope, followed by culture of the oocyte for 2 days. Follicles containing granulosa cells which failed to attach and grow, or degenerated oocytes (at the time of harvest or degenerated in culture) were not used and were designated atretic. After initiation of the LH surge, there was a significant rise in follicular fluid progesterone and estrogen levels, with estrogen levels reaching a peak at the time of the LH surge. One day after the LH surge, follicular fluid and serum estrogen levels dropped sh...