The influence of suspension nebulization or instillation on particle uptake by guinea pig alveolar macrophages.
The influence of suspension nebulization or instillation on particle uptake by guinea pig alveolar macrophages.
复制标题
悬浮雾化或滴注对豚鼠肺泡巨噬细胞颗粒摄取的影响。
DOI：
10.1080/08958370120643
复制
发表时间：
2001
影响因子：
2.1
通讯作者：
Hickey,AJ
中科院分区：
文献类型：
--
作者：
Suarez,S;Kazantseva,M;Bhat,M;Costa,D;Hickey,AJ
吞噬作用是宿主防御病原体的关键事件。需要允许输送一定范围的颗粒剂量的实验方法。然而，尚不清楚这些方法是否会导致相同的沉积位点或清除机制。研究了雾化或滴注颗粒给药对豚鼠肺泡巨噬细胞 (AM) 吸收的影响。通过雾化（1.4×107-11.1×107 颗粒）或滴注（19×107-152×107 颗粒）15 分钟将逐渐增加的 1 µm 荧光聚苯乙烯乳胶微球的悬浮液输送到豚鼠的肺部。选择这些剂量以最大化使用这些方法中的每一种的递送。给药后6小时通过肺泡灌洗收集巨噬细胞。回收细胞总数为3×106，台盼蓝排除法测定细胞活力>97%。对灌洗细胞悬浮液进行分类细胞计数，结果显示两种施用方法与不同浓度的乳胶颗粒和对照样品没有差异。使用落射荧光、共聚焦显微镜和流式细胞术测量颗粒的摄取。通过显微镜和流式细胞术测量，AMs 显示出相关颗粒的剂量依赖性增加。噬菌体中存在直接相关性（R2=.99）
Phagocytosis represents a crucial event in the host defense against pathogens. Experimental methods are required that allow a range of particle doses to be delivered. However, it is not clear that these methods result in the same sites of deposition or mechanisms of clearance. The effect of particle administration by nebulization or instillation on the uptake by guinea pig alveolar macrophages (AMs) has been studied. Suspensions of escalating quantities of 1-µm fluorescent polystyrene latex microspheres were delivered by 15 min of nebulization (1.4× 107–11.1× 107 particles) or instillation (19× 107–152× 107 particles) into the lungs of guinea pigs. These doses were selected to maximize delivery using each of these methods. Macrophages were collected by alveolar lavage 6 h postadministration. The total number of cells recovered was 3× 106 and the cell viability was> 97%, which was measured by trypan blue exclusion. Differential cell counts of lavaged cell suspensions were conducted and results showed no difference for the two methods of administration with various concentrations of latex particles and control samples. The uptake of particles was measured using epifluorescence, confocal microscopy, and flow cytometry. AMs showed a dose-dependent increase in associated particles measured by microscopy and flow cytometry. There was a direct correlation (R2=. 99) in the phago-