Suppressive effects of sulfated polysaccharide ascophyllan isolated from Ascophyllum nodosum on the production of NO and ROS in LPS-stimulated RAW264.7 cells
Suppressive effects of sulfated polysaccharide ascophyllan isolated from Ascophyllum nodosum on the production of NO and ROS in LPS-stimulated RAW264.7 cells
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DOI：
10.1093/bbb/zbaa115
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发表时间：
2021-03-24
影响因子：
1.6
通讯作者：
Oda, Tatsuya
中科院分区：
文献类型：
--
作者：
Liang, Yan;Zha, Shijiao;Oda, Tatsuya
在这项研究中，我们发现一个硫酸化多糖分离的棕色Ascophyllum nodosum，Ascophyllan，对刺激的RAW 264.7细胞表现出抑制作用。在脂多糖（LPS）刺激的RAW264.7细胞中，紫珠多糖以剂量依赖的方式显著抑制诱导型一氧化氮合酶mRNA的表达和一氧化氮（NO）的过量产生，而不影响RAW264.7细胞的活力。在LPS刺激的RAW264.7细胞中，Aspophyllan还降低了细胞内活性氧（ROS）的升高水平。此外，与Ascophyllan预孵育导致佛波醇12-肉豆蔻酸酯-13-乙酸酯刺激的RAW 264.7细胞中ROS产生的浓度依赖性降低。我们的研究结果表明，Ascophyllan可以表现出对刺激的巨噬细胞的抗炎作用，主要是通过减少NO和ROS的产生。
In this study, we found that a sulfated polysaccharide isolated from the brown alga Ascophyllum nodosum, ascophyllan, showed suppressive effects on stimulated RAW264.7 cells. Ascophyllan significantly inhibited expression of inducible nitric oxide synthase mRNA and excessive production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells in a dose-dependent manner without affecting the viability of RAW264.7 cells. Ascophyllan also reduced the elevated level of intracellular reactive oxygen species (ROS) in LPS-stimulated RAW264.7 cells. Furthermore, preincubation with ascophyllan resulted in concentration-dependent decrease in ROS production in phorbol 12-myristate-13-acetate-stimulated RAW264.7 cells. Our results suggest that ascophyllan can exhibit anti-inflammatory effects on stimulated macrophages mainly through the attenuation of NO and ROS productions.