Comparison of lumbar spine fusion using mixed and cloned marrow cells
Comparison of lumbar spine fusion using mixed and cloned marrow cells
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DOI：
10.1097/00007632-200111010-00003
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发表时间：
2001-11-01
期刊：
影响因子：
3
通讯作者：
Wang, GJ
中科院分区：
文献类型：
--
作者：
Cui, QJ;Xiao, ZM;Wang, GJ
研究设计。应用克隆和混合骨髓细胞进行腰椎融合的前瞻性研究。使用可追踪的基因分析克隆的骨祖细胞在脊柱融合中的有效性及其体内分化。背景数据摘要。虽然自体植骨目前是稳定脊柱融合的标准，但供应有限。需要开发和评估替代的移植物材料。将从小鼠骨髓中克隆并转导LacZ和新霉素耐药基因的成骨细胞(DL-Bag)和来自骨髓喷口的混合骨髓基质细胞用于无菌大鼠的后路脊柱融合，36只动物将100-Matrigel中的2×10(6)细胞植入腰椎融合床，16只动物作为对照。分别于2、3、6、9周处死大鼠，通过人工触诊、X线片和组织学方法评价脊柱。术后两周，在植入D1-Bag细胞的移植部位可见不透射线的组织，但在植入混合骨髓基质细胞的部位未见放射不透明组织。接受DI-Bag细胞移植的8只动物中有8只(100%)在6周和9周时成功融合，在混合骨髓基质细胞移植的8只动物中有4只(50%)获得成功融合，在对照组8只动物中仅有0只(0%)。与混合骨髓基质细胞相比，克隆的骨祖细胞可以在脊柱融合的早期时间点产生更多的成熟骨组织。
Study Design. Prospective study on lumbar spine fusion using cloned and mixed marrow cells.Objective. To analyze the effectiveness of cloned osteoprogenitor cells in spine fusion and their differentiation in vivo using a traceable gene.Summary of Background Data. Although autografts are currently the standard for stable spine fusion, supply, is limited. Alternative graft materials need to be developed and evaluated.Methods. An osteoprogenitor cell, Dl-BAG, cloned from mouse bone marrow and transduced with LacZ and neomycin resistance genes, and mixed marrow stromal cells from marrow blowouts were used in athymic rats to establish posterior spinal fusion; 2 x 10(6) cells in 100 muL Matrigel were implanted into the lumbar fusion bed in 36 animals, whereas Matrigel without cells was used in 16 animals as control. Rats were killed at 2, 3, 6, and 9 weeks, and the spines were evaluated by manual palpation, radiographs, and histology.Results. Two weeks after surgery radiopaque tissue was seen at transplantation sites with D1-BAG cells but not at sites with mixed marrow stromal cells. Successful spine fusion at 6 and 9 weeks was observed in 8 of 8 (100%) animals receiving DI-BAG cells, 4 of 8 (50%) in mixed marrow stromal cells, and 0 of 8 (0%) in control animals.Conclusions. Compared with mixed marrow stromal cells, cloned osteoprogenitor cells can produce a larger amount of mature osseous tissue at an earlier time point during spine fusion.