Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria
Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria
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DOI：
10.1016/j.jphotobiol.2020.111903
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发表时间：
2020-08-01
影响因子：
5.4
通讯作者：
Fontana, Carla Raquel
中科院分区：
文献类型：
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作者：
de Paula Zago, Lucas Henrique;de Annunzio, Sarah Raquel;Fontana, Carla Raquel
抗菌光动力疗法（aPDT）作为一种替代且有前途的消毒方法脱颖而出，并已被用于治疗口腔细菌。在这项研究中，我们在体外评估了亚甲蓝、二氢卟酚-e6 和姜黄素介导的 aPDT 对甲硝唑耐药的临床龈下菌斑的作用。采用琼脂稀释法分析样品对甲硝唑的敏感性。通过 CFU/mL 评估浮游和生物膜阶段的细胞活力。通过棋盘DNA-DNA杂交技术评估生物膜的组成。通过共焦荧光显微镜（CLSM）定性评估光敏剂内化。由所测试的三种光敏剂介导的 aPDT 能够减少浮游微生物负荷的总量并部分减少生物膜样本。 CLSM 进行的分析表明，aPDT 应用中使用的光敏剂能够渗透到生物膜的内部。 aPDT 已被证明可作为支持性且有效的牙周病治疗方法。
The antimicrobial photodynamic therapy (aPDT) has stood out as an alternative and promising method of disinfection and has been exploited for the treatment of oral bacteria. In this study, we evaluate in vitro the action of aPDT, mediated by methylene blue, chlorin-e6, and curcumin against clinical subgingival plaques that were resistant to metronidazole. The sensitivity profile of the samples to metronidazole was analyzed by the agar dilution method. Cell viability in the planktonic and biofilm phase was assessed by CFU / mL. The composition of the biofilm was evaluated by the checkboard DNA-DNA Hibrydization technique. Photosensitizers internalization was qualitatively assessed by confocal fluorescence microscopy (CLSM). The aPDT mediated by the three photosensitizers tested was able to reduce the totality of the planktonic microbial load and partially reduce the biofilm samples. The analysis performed by CLSM showed that the photosensitizers used in the application of aPDT were able to permeate the interior of the biofilm. The aPDT has been shown to be useful in a supportive and effective approach to the treatment of periodontal disease.