Microbes Promote Amino Acid Harvest to Rescue Undernutrition in Drosophila.
Microbes Promote Amino Acid Harvest to Rescue Undernutrition in Drosophila.
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DOI：
10.1016/j.celrep.2015.01.018
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发表时间：
2015-02-17
期刊：
影响因子：
8.8
通讯作者：
Ja WW
中科院分区：
文献类型：
--
作者：
Yamada R;Deshpande SA;Bruce KD;Mak EM;Ja WW
微生物在蛋白质特异性营养不良等营养性疾病的发病机制中起着重要作用。然而，在营养不良期间，微生物对宿主能量平衡的确切贡献尚不清楚。在这里，我们展示了一种从野外捕获的黑腹果蝇中分离出来的真菌微生物Issatchenkia orientalis，它可以促进氨基酸的收集，从而挽救营养不良的果蝇的寿命。使用放射性同位素标记的饮食成分(氨基酸、核苷酸和蔗糖)来量化从食物到苍蝇微生物的营养转移，我们证明了东方飞虱直接从营养贫乏的饲料中提取氨基酸，并增加了对苍蝇的蛋白质通量。这种微生物组合恢复了体重、蛋白质、甘油和三磷酸腺苷水平，并显示了喂食充足的果蝇的代谢特征。我们的研究揭示了氨基酸收获是连接微生物和宿主代谢的基本机制，并强调了果蝇作为定量研究宿主-微生物关系的平台。
Microbes play an important role in the pathogenesis of nutritional disorders such as protein-specific malnutrition. However, the precise contribution of microbes to host energy balance during undernutrition is unclear. Here, we show that Issatchenkia orientalis, a fungal microbe isolated from field-caught Drosophila melanogaster, promotes amino acid harvest to rescue the lifespan of undernourished flies. Using radioisotope-labeled dietary components (amino acids, nucleotides, and sucrose) to quantify nutrient transfer from food to microbe to fly, we demonstrate that I. orientalis extracts amino acids directly from nutrient-poor diets and increases protein flux to the fly. This microbial association restores body mass, protein, glycerol, and ATP levels and phenocopies the metabolic profile of adequately fed flies. Our study uncovers amino acid harvest as a fundamental mechanism linking microbial and host metabolism, and highlights Drosophila as a platform for quantitative studies of host-microbe relationships.