Optotheranostic Nanosystem with Phone Visual Diagnosis and Optogenetic Microbial Therapy for Ulcerative Colitis At-Home Care
Optotheranostic Nanosystem with Phone Visual Diagnosis and Optogenetic Microbial Therapy for Ulcerative Colitis At-Home Care
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具有电话视觉诊断和光遗传学微生物疗法的光治疗纳米系统用于溃疡性结肠炎家庭护理
DOI：
10.1021/acsnano.1c00135
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发表时间：
2021-04-05
期刊：
影响因子：
17.1
通讯作者：
Wang, Hanjie
中科院分区：
文献类型：
--
作者：
Cui, Meihui;Pang, Gaoju;Wang, Hanjie
溃疡性结肠炎(UC)是一种以肠道慢性炎症为特征的复发性疾病。然而，基于远程监控的UC家庭护理，由于操作复杂、程序耗时，制约了其广泛应用。在这里，我们构建了一个光热疗纳米系统，用于家庭中UC的自我诊断和长效缓解。该系统包括两个主要模块：(I)基于智能手机光学传感的疾病预筛模块。(Ii)由光基因工程细菌系统介导的疾病实时干预模块。分泌白介素10(IL-10)的重组大肠杆菌Nissle1917(ECN)可下调炎性级联反应和基质金属蛋白酶的表达，有望用于UC的治疗干预。结果表明，该检测器能够在不到1min的时间内对检测结果进行分析、报告和共享，检测限为15ng.mL(-1)。此外，分泌IL-10的ECN治疗可抑制UC小鼠的肠炎症反应，保护肠粘膜免受损伤。光疗纳米系统使解决方案能够在家中诊断和治疗疾病，这促进了移动医疗服务的发展。
Ulcerative colitis (UC) is a relapsing disorder characterized by chronic inflammation of the intestinal tract. However, the home care of UC based on remote monitoring, due to the operational complexity and time-consuming procedure, restrain its widespread applications. Here we constructed an optotheranostic nanosystem for self-diagnosis and long-acting mitigations of UC at home. The system included two major modules: (i) A disease prescreening module mediated by smartphone optical sensing. (ii) Disease real-time intervention module mediated by an optogenetic engineered bacteria system. Recombinant Escherichia coli Nissle 1917 (EcN) secreted interleukin-10 (IL-10) could down-regulate inflammatory cascades and matrix metalloproteinases; it is a candidate for use in the therapeutic intervention of UC. The results showed that the Detector was able to analyze, report, and share the detection results in less than 1 min, and the limit of detection was 15 ng.mL(-1). Besides, the IL-10-secreting EcN treatment suppressed the intestinal inflammatory response in UC mice and protected the intestinal mucosa against injury. The optotheranostic nanosystems enabled solutions to diagnose and treat disease at home, which promotes a mobile health service development.