HEMATOPORPHYRIN-SENSITIZED PHOTOINACTIVATION OF STREPTOCOCCUS-FAECALIS
HEMATOPORPHYRIN-SENSITIZED PHOTOINACTIVATION OF STREPTOCOCCUS-FAECALIS
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DOI：
10.1111/j.1751-1097.1984.tb08864.x
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发表时间：
1984-01-01
影响因子：
3.3
通讯作者：
JORI, G
中科院分区：
文献类型：
--
作者：
BERTOLONI, G;SALVATO, B;JORI, G
本文报道了血卟啉（HP）对S.粪球菌，金黄色葡萄球菌，大肠杆菌和肺炎克雷伯氏菌和白色念珠菌酵母菌株，在空气平衡的水介质中，在pH 7.2进行了研究。所有的革兰氏阴性菌株似乎是耐甚至延长照射的存在和不存在的EDTA。革兰氏阳性和酵母菌株是光敏的。在S. faecalis，其菌株表现出特别高的光敏性，光过程的效率主要由孵育培养基中HP浓度（0.1-1 μ g/ml）和照射时间（0-10分钟）、温度（18 ℃-15 ℃）控制。37.degree. C）产生较小的影响，而孵育时间（6-30分钟）没有可检测到的影响。S.粪菌细胞仅在生长的对数期和稳定期略有不同。的类型的光诱导的超微结构的修改和HP的细胞质膜，观察到的细胞分级分离的研究，在很大程度上优先结合，表明一个重要的目标的HP光敏化过程是由膜组件。
The photosensitizing action of hematoporphyrin (HP) on the cells of some bacterial strains belonging to the species S. faecalis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and strains of yeast Candida albicans, was studied in air-equilibrated aqueous media at pH 7.2. All gram-negative strains appeared to be resistant even to prolonged irradiation in the presence and in the absence of EDTA. Gram-positive and yeast strains were photosensitive. In the case of S. faecalis, whose strains displayed a particularly high photosensitivity, the efficiency of the photoprocess was mainly controlled by the HP concentration in the incubation medium (0.1-1 .mu.g/ml) and by the irradiation time (0-10 min); temperature (18.degree.-37.degree. C) exerted a minor effect, whereas the incubation time (6-30 min) had no detectable influence. The photolability of S. faecalis cells was only slightly different in the logarithmic and stationary phases of growth. The type of photoinduced ultrastructural modifications and the largely preferential binding of HP to the cytoplasmic membrane, as observed by cell fractionation studies, suggest that one important target of the HP-photosensitized process is represented by membrane components.