Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp.
Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp.
复制标题
DOI：
10.1046/j.1365-2672.2003.02064.x
复制
发表时间：
2003-01-01
影响因子：
4
通讯作者：
Borgmann-Strahsen, R
中科院分区：
文献类型：
--
作者：
Langsrud, S;Sundheim, G;Borgmann-Strahsen, R
目的：确定用于消毒剂测试的菌株（铜绿假单胞菌 ATCC 15442）和食品相关分离株对苯扎氯铵和二癸基二甲基氯化铵 (DDAC) 的敏感性。确定适应 DDAC 后细菌耐药性的增加是否与表型变化相关。测试替代消毒剂消除耐药假单胞菌的活性。方法和结果：铜绿假单胞菌 ATCC 15442 是使用杀菌悬浮液测试测试的耐药性最强的菌株之一。敏感诗篇的成长。逐渐升高浓度的 DDAC 中的荧光菌导致对几种抗菌剂产生稳定的较高耐药性和一些交叉耐药性，但含有氯胺 T、戊二醛或过氧乙酸的消毒剂除外。显微镜显示，适应之后会出现鞭毛消失和粘液形成。用十二烷基硫酸钠去除粘液导致获得性抗性部分丧失。结论：假单胞菌属。细菌可能会适应较高浓度的季铵化合物 (QAC) 的生存，但使用化学上不相关的消毒剂可以消除耐药菌株。这项研究的意义和影响：这项工作支持在食品加工环境中轮换消毒剂，以避免细菌对 QAC 产生耐药性。由于可能存在交叉耐药性，应谨慎选择交替使用的消毒剂。
Aims: To determine the sensitivity of a strain used for disinfectants testing (Pseudomonas aeruginosa ATCC 15442) and food-associated isolates to benzalkonium chloride and didecyl dimethylammonium chloride (DDAC). To determine whether the increase in bacterial resistance after adaptation to DDAC can be associated with phenotypic changes. To test the activity of alternative disinfectants to eliminate resistant Pseudomonas spp.Methods and Results: Pseudomonas aeruginosa ATCC 15442 was among the most resistant strains tested using a bactericidal suspension test. Growth of a sensitive Ps. fluorescens in gradually higher concentrations of DDAC resulted in stable higher resistance and to some cross-resistance to several antibacterial agents, with the exception of disinfectants containing chloramine T, glutaraldehyde or peracetic acid. It was shown by microscopy that adaptation was followed by loss of flagella, and slime formation. Removal of the slime by sodium dodecyl sulphate resulted in partial loss of the acquired resistance.Conclusions: Pseudomonas spp. may adapt to survive against higher concentrations of quaternary ammonium compounds (QACs), but resistant strains can be eliminated with chemically unrelated disinfectants.Significance and Impact of the Study: The work supports the rotation of disinfectants in food processing environments for avoiding the development of bacterial resistance to QACs. The alternating disinfectants should be chosen carefully, because of possible cross-resistance.