Trichosanthin inhibits the proliferation of cervical cancer cells and downregulates STAT-5/C-myc signaling pathway
Trichosanthin inhibits the proliferation of cervical cancer cells and downregulates STAT-5/C-myc signaling pathway
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DOI：
10.1016/j.prp.2018.12.010
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发表时间：
2019-04-01
影响因子：
2.8
通讯作者：
Zheng, Ai
中科院分区：
文献类型：
--
作者：
Chen, Yali;Han, Ling;Zheng, Ai
背景资料：研究表明天花粉蛋白具有抗病毒、免疫调节和广谱抗肿瘤等药理活性。天花粉蛋白是一种很有前途的治疗宫颈癌的药物。方法：采用CCK-8法检测TCS对宫颈癌细胞株HeLa和caski的细胞活力。Ki-67染色检测细胞增殖，流式细胞仪检测细胞凋亡。创伤实验和transwell实验分别检测细胞迁移和侵袭能力。结果：TCS能抑制HeLa细胞和caski细胞增殖，降低Ki-67和P-C-myc的表达。流式细胞仪检测结果显示TCS可诱导HeLa细胞和caski细胞凋亡。TCS对细胞凋亡的有效作用通过增加caspase-3水平和降低Bcl-2水平来确定。TCS还抑制宫颈癌细胞的侵袭、迁移和上皮间质转化（EMT）。结论：TCS抑制人宫颈癌细胞的增殖、迁移和EMT，其作用机制可能与抑制STAT 5/C-myc信号通路有关。
Background: Previous studies have indicated that Trichosanthin (TCS) exerts anti-virus, immunoregulation and a broad spectrum anti-tumor pharmacological activities. Trichosanthin is a promising agent for the treatment of cervical cancer. However, the exact effects and potential mechanism of TCS on cervical cancer are not well known.Method: The cell viability of TCS on cervical cancer cell lines (HeLa and caski cells) were detected by a Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was measured by Ki-67 staining and cell apoptosis was detected by flow cytometry. Cell migration and invasion were detected by wound assay and transwell assay, respectively. The levels of E-cadherin, N-cadherin, Snail, Bcl-2, Caspase-3, p-STATS, STAT5, p-C-myc, C-myc were detected by western blot.Results: The present study showed that TCS inhibited the proliferation of HeLa and caski cells and reduced Ki-67 and P-C-myc expression. In addition, flow cytometric analysis showed that TCS induced the apoptosis of HeLa and caski cells. The potent effect of TCS on cell apoptosis as determined by the increase the levels of caspase-3 and decrease the levels of Bcl-2. TCS also inhibited cervical cancer cell invasion, migration and epithelial-mesenchymal transition (EMT). Furthermore, TCS treatment markedly inhibited the activation of STAT5/C-myc signaling pathway.Conclusion: In conclusion, the present study suggest that TCS inhibits the proliferation, migration and EMT of human cervical cancer cells, which maybe mediated by inhibiting the activation of STAT5/C-myc signaling pathway.