Tumor-associated lymphatic endothelial cell promotes invasion of cervical cancer cells
Tumor-associated lymphatic endothelial cell promotes invasion of cervical cancer cells
复制标题
肿瘤相关淋巴管内皮细胞促进宫颈癌细胞侵袭
DOI：
10.1111/apm.12068
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发表时间：
2013-12-01
期刊：
影响因子：
2.8
通讯作者：
Wang, Zehua
中科院分区：
文献类型：
--
作者：
Cai, Liqiong;Yang, Shouhua;Wang, Zehua
宫颈癌最常见的传播方式是通过淋巴系统。肿瘤相关淋巴管内皮细胞(TLEC)在某些肿瘤的转移中起着至关重要的作用。本研究的目的是从人宫颈癌组织中分离TLEC，并探讨其在体外参与转移相关行为的作用。应用D2-40抗体免疫组织化学染色检测62例宫颈组织标本中淋巴管的分布。分析淋巴管密度(LVD)与临床病理特征的关系。用LYVE-1免疫磁珠从宫颈癌组织中分离出原代TLEC，并通过LEC标志物Prox-1和D2-40的表达进行验证，然后进行体外培养。Transwell法检测细胞侵袭力，台盼蓝拒染法检测细胞活性。我们的结果表明，高LVD与晚期FIGO分期、盆腔淋巴结转移(LNM)和细胞分化不良显著相关。成功地进行了TLECs的初步分离和体外培养。TLEC培养上清可增强Hela细胞的侵袭力，但对细胞存活率无明显影响。结论：TLECs可能对宫颈癌的淋巴转移有积极的促进作用。还需要进一步的研究来证明其潜在的机制。
The most common way for cervical cancer to spread is through the lymphatic system. Tumor-associated lymphatic endothelial cell (TLEC) has been considered to play a crucial role in metastasis of certain cancers. The aim of this study was to isolate TLEC from human cervical cancers and explore its involvement in metastasis-associated behaviors in vitro. Lymphatic vessels in 62 cervix tissue specimens ranging from cervical intraepithelial neoplasia (CIN) to advanced invasive cancer were detected using immunochemical staining with D2-40 antibody. Relation of lymphatic vessel density (LVD) to clinicopathological characters was analyzed. Primary TLECs were isolated by LYVE-1 immuno-magnetic beads from cervical cancer tissues and verified through expression of LEC markers Prox-1 and D2-40, and then cultured in vitro. Invasiveness and viability of cells were assessed by transwell assay and typan blue exclusion, respectively. Our results showed that higher LVD was significantly associated with advanced FIGO stage, pelvic lymphatic nodal metastasis (LNM), and poorer cell differentiation. TLECs were successfully primarily isolated and cultured in vitro. Supernatant of TLEC enhanced invasiveness of Hela cell, but did not significantly affect cell viability. In conclusion, TLECs might actively promote lymphatic metastasis of cervical cancer. Further studies are needed to demonstrate the underlying mechanisms.