Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue
Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue
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DOI：
10.1007/978-1-4939-1242-1_14
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发表时间：
2014-01-01
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影响因子：
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通讯作者：
Bobola, Nicoletta
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文献类型：
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作者：
Amin, Shilu;Bobola, Nicoletta
在胚胎发育过程中必须严格控制基因表达的调节。控制基因表达的中心机制是序列特异性转录因子与基因组中顺式调控元件的结合。染色质免疫沉淀（ChIP）是一种广泛使用的技术，用于分析转录因子的结合和染色质上的组蛋白修饰;然而，它仅限于观察少数基因。大规模并行测序技术（ChIP-seq）是近年来发展起来的一种分析转录因子与组蛋白修饰的全基因组结合的有力工具，为基因表达调控提供了大量信息。本章介绍了如何在小鼠胚胎组织上进行ChIP和ChIP-seq。
Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone modifications on chromatin; however, it is limited to looking at a small number of genes. ChIP with massively parallel sequencing (ChIP-seq) is a recently developed powerful tool to analyze genome-wide binding of transcription factors and histone modifications and provides a vast amount of information into the regulation of gene expression. This chapter describes how ChIP and ChIP-seq are performed on mouse embryonic tissue.