Effect of CPP-ACP paste on mechanical properties of bovine enamel as determined by an ultrasonic device
Effect of CPP-ACP paste on mechanical properties of bovine enamel as determined by an ultrasonic device
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DOI：
10.1016/j.jdent.2005.06.005
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发表时间：
2006-03-01
影响因子：
4.4
通讯作者：
Moore, BK
中科院分区：
文献类型：
--
作者：
Yamaguchi, K;Miyazaki, M;Moore, BK
目的：本研究的目的是确定酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）糊剂对牛牙釉质脱矿的影响，通过测量超声传输速度的变化。将标本保存在0.1M的tactic acid缓冲液（pH4.75，Ca0.75mM，P0.45mM）中10 min，每日2次，然后保存在人工唾液（pH7.0）中。其他标本在CPP-ACP糊剂和不含CPP-ACP的安慰剂糊剂的10倍稀释溶液中储存10分钟，然后在人工唾液中储存前每天两次浸入脱矿溶液中10分钟。纵向超声波的传播时间通过具有换能器（V112，Panametrics）的脉冲发生器-接收器（5900型，Panametrics）测量。每个条件下使用6个标本，然后由Tukey HSD测试（α =0.05）的单因素方差分析weredes.Results：声速被发现随着时间的推移，储存在脱矿质溶液中的标本减少。另一方面，一个显着的增加声速被发现为标本存储在CPP-ACP solution.Conclusions：从本研究的结果，它建议的脱矿和矿化的釉质结构的条件，可以测量非破坏性地通过使用超声波脉冲方法。由此可见，CPP-ACP中高浓度的无机成分对釉质结构的矿化有促进作用。(C)2005爱思唯尔有限公司保留所有权利。
Objective: The purpose of this study was to determine the effect of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) paste on demineralization of bovine enamel by measuring changes in the ultrasound transmission velocity.Methods: The enamel specimens were prepared by cutting bovine teeth into blocks. The specimens were stored in 0.1 M tactic acid buffer solution (pH 4.75, Ca 0.75 mM, P 0.45 mM) for 10 min twice a day, and then stored in the artificial saliva (pH 7.0). Other specimens were stored in a 10-times diluted solution of CPP-ACP paste and a placebo paste without CPP-ACP for 10 min, followed by 10 min immersion into a demineralization solution twice a day before storage in the artificial saliva. The propagation time of longitudinal ultrasonic waves was measured by a Pulser-Receiver (Model 5900, Panametrics) with a transducer (V112, Panametrics). Six specimens were used for each condition, and one-way ANOVAs followed by the Tukey HSD tests (alpha=0.05) were done.Results: The sonic velocity was found to decrease with time for specimens stored in the demineralization solution. On the other hand, a significant increase in sonic velocity was found for specimens stored in the CPP-ACP solution.Conclusions: From the result of this study, it was suggested that the conditions of de- and remineralization of the enamel structure could be measured non-destructively by using an ultrasonic pulse method. It could be concluded that the inorganic components contained in high concentrations in CPP-ACP acted to enhance remineralization of the enamel structure. (C) 2005 Elsevier Ltd. All rights reserved.