Biodegradation and chemotaxis of polychlorinated biphenyls, biphenyls, and their metabolites by Rhodococcus spp.
Biodegradation and chemotaxis of polychlorinated biphenyls, biphenyls, and their metabolites by Rhodococcus spp.
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DOI：
10.1007/s10532-017-9809-6
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发表时间：
2018-02
期刊：
影响因子：
3.6
通讯作者：
Hui Wang;Jinxing Hu;Kai Xu;Xianjin Tang;Xinhua Xu;Chaofeng Shen
中科院分区：
文献类型：
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作者：
Hui Wang;Jinxing Hu;Kai Xu;Xianjin Tang;Xinhua Xu;Chaofeng Shen
从多氯联苯污染土壤中分离到2株联苯降解菌SS1和SS2。根据16S rRNA基因序列及形态、生理生化特征鉴定为红色红球菌和吡啶红球菌。SS1和SS2对2000和3000 mg/L的联苯表现出耐受性。在84h内对1300 mg/L联苯的降解率分别为83.2%和71.5%。在低氯化多氯联苯同系物、苯甲酸酯和3-氯苯甲酸酯的情况下，SS1和SS2的降解活性也很显著。此外，这两株菌对三氯乙酸环中间体苯甲酸酯、联苯和2-氯苯甲酸酯表现出趋化反应。这项研究表明，与鞭毛化细菌一样，非鞭毛化的红球菌也是如此。一旦底物在其周围耗尽，可能会通过趋化作用积极地寻找底物。总之，这些数据提供了支持证据，表明SS1和SS2可能是恢复联苯/多氯联苯污染环境的良好候选者。
Two biphenyl-degrading bacterial strains, SS1 and SS2, were isolated from polychlorinated biphenyl (PCB)-contaminated soil. They were identified asRhodococcus ruberandRhodococcus pyridinivoransbased on the 16S rRNA gene sequence, as well as morphological, physiological and biochemical characteristics. SS1 and SS2 exhibited tolerance to 2000 and 3000 mg/L of biphenyl. And they could degrade 83.2 and 71.5% of 1300 mg/L biphenyl within 84 h, respectively. In the case of low-chlorinated PCB congeners, benzoate and 3-chlorobenzoate, the degradation activities of SS1 and SS2 were also significant. In addition, these two strains exhibited chemotactic response toward TCA-cycle intermediates, benzoate, biphenyl and 2-chlorobenzoate. This study indicated that, like the flagellated bacteria, non-flagellatedRhodococcusspp. might actively seek substrates through the process of chemotaxis once the substrates are depleted in their surroundings. Together, these data provide supporting evidence that SS1 and SS2 might be good candidates for restoring biphenyl/PCB-polluted environments.