Innervation of the heart of the adult fruit fly, Drosophila melanogaster
Innervation of the heart of the adult fruit fly, Drosophila melanogaster
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DOI：
10.1002/cne.10869
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发表时间：
2003-10-27
影响因子：
2.5
通讯作者：
Levine, RB
中科院分区：
文献类型：
--
作者：
Dulcis, D;Levine, RB
采用绿色荧光蛋白染色和免疫细胞化学技术对黑腹果蝇成虫腹心的神经支配进行了研究。这项研究是为了确定成人心脏是否接受神经元输入，或者它的复杂活动是否必须独立于神经系统。幼体心脏缺乏神经支配，表明心脏冲动完全是肌源性的。变态时，节段性神经突生长在心肌上。一对横神经支配双侧的每个心腔及其侧肌。这些神经末梢对谷氨酸有免疫反应，在锥形室纵心肌腹侧层形成独特的突触结构。这种特征性的心脏突触可能代表了控制逆行心跳的部分神经机制，因此，心脏逆转是成年人的特征。此外，源自外周双极神经元（BpNs）的甲壳类心脏活性肽免疫反应纤维与横神经突起一起束状，并在整个腹部心脏中节段性地终止。另一个由四个大的cca -p阳性神经元组成的簇支配着终末室。在这个位置释放CCAP的加速作用可以调节起搏器产生顺行性心跳的特性。[j] .中国生物医学工程学报，2016,35(5):557 - 557。(C) 2003 Wiley-Liss, Inc。
The innervation of the adult abdominal heart of Drosophila melanogaster was studied by neuronal staining with green fluorescent protein and immunocytochemical techniques. The investigation was undertaken to determine whether the adult heart receives neuronal input or whether its complex activity must be considered independent from the nervous system. The larval heart lacks innervation, suggesting that the cardiac impulse is totally myogenic. At metamorphosis, segmental neural processes grow onto the myocardium. A pair of transverse nerves innervates bilaterally each cardiac chamber and its alary muscles. These nerve terminals are immunoreactive to glutamate and form unique synaptic structures on the ventral layer of longitudinal cardiac muscles of the conical chamber. This characteristic cardiac synapse may represent part of the neural mechanism controlling the retrograde heartbeat, and, thus, the cardiac reversal that is characteristic of adults. In addition, crustacean cardioactive peptide-immunoreactive fibers originating from peripheral, bipolar neurons (BpNs) fasciculate with the transverse nerve projections and terminate segmentally throughout the abdominal heart. An additional cluster composed of four large, CCA-P-positive neurons innervates the terminal chamber. The cardioacceleratory effect of CCAP release at this location may modulate the properties of a pacemaker producing the anterograde heartbeat. J. Comp. Neurol. 465:560-578, 2003. (C) 2003 Wiley-Liss, Inc.