Mechanics of fresh, refrigerated, and frozen arterial tissue
Mechanics of fresh, refrigerated, and frozen arterial tissue
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DOI：
10.1016/j.jss.2006.09.001
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发表时间：
2007-05-15
影响因子：
2.2
通讯作者：
Pintar, Frank A.
中科院分区：
文献类型：
--
作者：
Stemper, Brian D.;Yoganandan, Narayan;Pintar, Frank A.
动脉移植物和实验软组织通常使用冷藏和冷冻来保存。本研究旨在探讨常用储存方案对动脉力学的影响。在新鲜、冷藏和冷冻储存条件下，猪椎间盘被轴向牵拉至失效。新鲜组织在处死后24小时内进行检测;冷冻组织在检测前在+4 ℃下储存24或48小时，冷冻组织在检测前在-20或-80 ℃下储存3个月。在82%的标本中，在最终失败之前，在牵张中发生了动脉钝性损伤伴内膜亚失效。与新鲜（0.83 +/- 0.39 MPa）和冷冻（0.99 +/- 0.41 MPa）样本相比，冷藏（0.59 +/- 0.19 MPa）样本的亚失效应力降低。与新鲜（1.15 +/- 0.39 MPa）和冷冻（1.32 +/-0.31 MPa）样本相比，冷藏（0.83 +/- 0.19 MPa）样本的极限应力也显著降低。亚失效和极限应变没有显着依赖于存储技术。与新鲜（2.98 +/- 1.45 MPa）和冷冻（3.49 +/- 1.32 MPa）样本相比，冷藏（1.89 +/- 0.63 MPa）样本的杨氏模量显著降低。新鲜和冷冻标本之间的生理，亚故障，和最终故障力学没有显着差异。临床相关内膜。在试验前遵循冷冻试样的实验方案，可以再现故障并确定损伤力学。然而，短期的组织冷冻可能会影响力学。(C)2007年爱思唯尔公司All rights reserved.
Arterial grafts and experimental soft tissues are commonly preserved using refrigeration and freezing. The present study was designed to investigate effects of common storage protocols on arterial mechanics. Porcine aortas were axially distracted to failure implementing fresh, refrigerated, and frozen storage conditions. Fresh tissues were tested within 24 h of sacrifice; refrigerated tissues were stored at +4 degrees C for 24 or 48 h prior to testing, and frozen tissues were stored at -20 or -80 degrees C for 3 months prior to testing. Blunt arterial injury experimentally occurred in distraction with intimal subfailure before ultimate failure in 82% of specimens. Subfailure stress decreased in refrigerated (0.59 +/- 0.19 MPa) compared to fresh (0.83 +/- 0.39 MPa) and frozen (0.99 +/- 0.41 MPa) specimens. Ultimate stress was also significantly decreased in refrigerated (0.83 +/- 0.19 MPa) compared to fresh (1.15 +/- 0.39 MPa) and frozen (1.32 +/- 0.31 MPa) specimens. Subfailure and ultimate strain were not significantly dependent upon storage technique. Young's modulus significantly decreased in refrigerated (1.89 +/- 0.63 MPa) compared to fresh (2.98 +/- 1.45 MPa) and frozen (3.49 +/- 1.32 MPa) specimens. Physiological, subfailure, and ultimate failure mechanics between fresh and frozen specimens were not significantly different. Clinically relevant intimal. failures can be reproduced and injury mechanics determined while adhering to experimental protocols of freezing specimens before testing. However, short-term tissue refrigeration may affect mechanics. (C) 2007 Elsevier Inc. All rights reserved.